CAJ | 학술논문

目的研究不同医院分离的鲍曼不动杆菌产碳青霉烯酶的基因型和分子流行特征.方法采用琼脂稀释法检测64株多重耐药鲍曼不动杆菌对抗菌药物的最小抑菌浓度(MIC);PCR检测碳青霉烯酶基因、整合酶基因及per基因,选取不同的耐药克隆菌株进行序列测定;脉冲场凝胶电泳(PFGE)分析菌株同源关系,确定菌株的分子流行特征.结果 50株菌(78.1%)携带blaOXA-23-like基因,经测序分析确定为OXA-23;1株菌检测出blaOXA-58-like基因;57株菌(89.1%)携带I类整合子;25株菌(39.1%)检测出blaPER-1基因.PFGE图谱显示64株菌分为A、B、C、D、E等13个基因型,三家医院分别以A型、B型和U型为主要流行株.结论三家医院均发现多耐药鲍曼不动杆菌的播散流行,不同医院间和同一医院不同科室间存在同型别流行;三家医院多耐药鲍曼不动杆菌产生的碳青霉烯酶常携带OXA-23型酶,同时检测出blaOXA-58、I类整合子和balPER基因.
목적 연구불동의원분리적포만불동간균산탄청매희매적기인형화분자류행특정.방법 채용경지희석법검측64주다중내약포만불동간균대항균약물적최소억균농도(MIC);PCR검측탄청매희매기인、정합매기인급per기인,선취불동적내약극륭균주진행서렬측정;맥충장응효전영(PFGE)분석균주동원관계,학정균주적분자류행특정.결과 50주균(78.1%)휴대blaOXA-23-like기인,경측서분석학정위OXA-23;1주균검측출blaOXA-58-like기인;57주균(89.1%)휴대I류정합자;25주균(39.1%)검측출blaPER-1기인.PFGE도보현시64주균분위A、B、C、D、E등13개기인형,삼가의원분별이A형、B형화U형위주요류행주.결론 삼가의원균발현다내약포만불동간균적파산류행,불동의원간화동일의원불동과실간존재동형별류행;삼가의원다내약포만불동간균산생적탄청매희매상휴대OXA-23형매,동시검측출blaOXA-58、I류정합자화balPER기인.
Objecfive To investigate antibiotic resistance,carbapenemase genotype and the molecular epidemiology of multidrug-resistant Acinetobacter baumannii (Aba) collected from 3 military hospitals in China.Methotis The minimum inhibitory concentrations (MIC) were examined by ager dilution method.Genotypes of carbapenemases were amplified by multiplex PCR and its products were sequenced.PCR was used to detect per gene,Homology of the resistant isolates was analyzed by pulse-field gel electrophoresis(PFGE).Results Among the 64 MDRA strains,78.1%(50)strains possessed blaOXA-23 gene,89.1%(57) carried Class 1 integrase gene,39.1% (25) with blaPER-1 gene,and 1 strain with blaOXA-58-like gene.PFGE showed that 13(A,B,C,D,E genotype) different clones were identified in these strains.A,B,and U clones were the predominant clones in three hospitals,respectitively.Conclusion OutbreaEs of muitidrug-rcsistant Aba occurred at 3 military hospitals with the most prevalent carbapenemase as OXA-23 enzyme.OXA-58 type of carbapenemase and per-1 in Aba were also isolated.