CAJ | 학술논문

目的评价长期吸入低浓度七氛烷对小鼠生殖功能的影响.方法 ICR雄性小鼠加只,日龄60 d,体重20～25 g,随机分为4组(n=10):对照组(C组);S1-3组分别吸入0.003%、0.01%和0.03%七氟烷.每天2 h,每周连续5 d,持续8周后处死动物.取睾丸和附皋,测定精子活动率、精子数量、精子畸变率、睾丸组织总乳酸脱氢酶(LDH)及乳酸脱氢酶同工酶-X(LDH-X)的活性,并观察睾丸组织超微结构.结果与C组比较,S3组精子活动率降低、畸变率升高、睾丸组织LDH-X活性降低(P<0.05),S1,2组各指标差异无统计学意义(P>0.05),仅S3组睾丸组织出现病理学改变.结论长期吸入0.03%七氟烷可导致雄性小鼠生殖功能异常,而长期吸入≤0.01%七氟烷对生殖功能未见影响.
목적 평개장기흡입저농도칠분완대소서생식공능적영향.방법 ICR웅성소서가지,일령60 d,체중20～25 g,수궤분위4조(n=10):대조조(C조);S1-3조분별흡입0.003%、0.01%화0.03%칠불완.매천2 h,매주련속5 d,지속8주후처사동물.취고환화부고,측정정자활동솔、정자수량、정자기변솔、고환조직총유산탈경매(LDH)급유산탈경매동공매-X(LDH-X)적활성,병관찰고환조직초미결구.결과 여C조비교,S3조정자활동솔강저、기변솔승고、고환조직LDH-X활성강저(P<0.05),S1,2조각지표차이무통계학의의(P>0.05),부S3조고환조직출현병이학개변.결론 장기흡입0.03%칠불완가도치웅성소서생식공능이상,이장기흡입≤0.01%칠불완대생식공능미견영향.
Objective To investigate rite effects of loag-term exposure to low-level sevoflurane on reproductive function in mice.Method F0ny male ICR mice,aged 60 d,weighlag 20-25 g,were randomly divided into 4 groups(n=10 each):control group received no sevoflunme(C);group S1-3 were exposed to 0.003%.0.01% and 0.03% sevoflurane 2 h per day for 5 consecutive days per week for 8 weeks respectively. The mice were then sacrificed at the end of the 8 weeks.The testes and epididymis were emoved and sampled for determination of the activities of total lactic dehydregenase(LDH)and lactic dehydrogenase-X(LDH-X),and the motility rate,amount,and aberration rate of sperm.Testicular uhrastructure were observed by transmission electron microscopy.Results The sperm motifity nne were significantly lower.the sperm aberration rate higher and the activity of LDH-X lower in group S3 than in group C(P<0.05),but there was no significant difference in the above parametem between group SI and group S2(P>0.05).The pathology changed of testes occurred only in group S3 among the 3 groups.Conclusion Long-term exposure to 0.03% sevoflurane can result in the abnormality of the reproductive function in male mice but exposure to≤0.01%sevoflurane dose not.