CAJ | 학술논문

目的观察分析遵义市汉族Eales病患者人类白细胞抗原-A/B、-DRB/DQB(HLA-A/B、DRB/DQB)基因的相关性,寻找可能的易感基因及保护基因. 方法 26例临床确诊为Eales病的遵义市汉族患者作为研究组,选取与研究组患者年龄、性别、民族等因素无差异的100名健康人为对照组.抽取外周静脉血,提取DNA,采用聚合酶链式反应特异序列引物法(PCR-SSP)检测HLA-A/B,HLA-DRB/DQB等位基因频率分布,计算两组优势比(OR). 结果 Eales病患者HLA-A*01(P=0.041,OR=20.5)-A*02(P=0.00;OR=54.667;OR95%CI为11.837-252.473)、-B*55(P=0.047;OR=4.524;OR95%CI为1.200-17.047),HLA-DRB*01(P=0.048;OR=5.879;OR95%CI为1.227-28.169),DQB*05(P=0.021,OR=2.769,OR95%CI为1.145-6.692)其分布频率明显高于对照组,差异有统计学意义(P<0.05),HLA-A*11(P=0.031,OR=0.383,OR95%CI为0.158-0.930)明显低于对照组,差异有统计学意义(P<0.05). 结论 Eales病可能存在遗传易感性,HLA-A*01,-A*02,-B*55,-DRB*01,-DQB*05可能是遵义市汉族Eales病患者的遗传易感基因,而HLA-A*11可能是该病的保护基因.
목적 관찰분석준의시한족Eales병환자인류백세포항원-A/B、-DRB/DQB(HLA-A/B、DRB/DQB)기인적상관성,심조가능적역감기인급보호기인. 방법 26례림상학진위Eales병적준의시한족환자작위연구조,선취여연구조환자년령、성별、민족등인소무차이적100명건강인위대조조.추취외주정맥혈,제취DNA,채용취합매련식반응특이서렬인물법(PCR-SSP)검측HLA-A/B,HLA-DRB/DQB등위기인빈솔분포,계산량조우세비(OR). 결과 Eales병환자HLA-A*01(P=0.041,OR=20.5)-A*02(P=0.00;OR=54.667;OR95%CI위11.837-252.473)、-B*55(P=0.047;OR=4.524;OR95%CI위1.200-17.047),HLA-DRB*01(P=0.048;OR=5.879;OR95%CI위1.227-28.169),DQB*05(P=0.021,OR=2.769,OR95%CI위1.145-6.692)기분포빈솔명현고우대조조,차이유통계학의의(P<0.05),HLA-A*11(P=0.031,OR=0.383,OR95%CI위0.158-0.930)명현저우대조조,차이유통계학의의(P<0.05). 결론 Eales병가능존재유전역감성,HLA-A*01,-A*02,-B*55,-DRB*01,-DQB*05가능시준의시한족Eales병환자적유전역감기인,이HLA-A*11가능시해병적보호기인.
Objective To analyze the association between histocompatibility leukocyte antigen (HLA-A/ B, HLA-DRB/DQB) alleles and Eales disease, and to explore susceptible genes and protective genes associated with Eales disease in a population of Han from ZUN YI city in Guizhou province. Methods The subjects were analyzed by case-control study consisted of two groups such as normal control group and Eales disease group. DNA samples from 100 healthy people and 26 patients with Eales disease were detected by polyrneriase chain reaction with sequence-specific primers (PCR-SSP). The alleles of HLA-A/B and HLA-DRB/DQB from Eales disease group were compared with those from control group by SPSS 13.0. Results The distribution frequency in Eales disease was HLA-A* 01(P=0.041, OR=20.5), -A*02(P=0.000, OR=54.667, OR95% CI: 11.837-252.473), -B * 55 (P=0.047, OR=4. 524;OR95% CI:1.200-17.047), HLA-DRB * 01 (P =0.048, OR=5.879, OR95%CI:1. 227-28.169). -DQB*05 (P=0.021, OR=2.769, OR95%CI=1.145-6.692) alleles, and obviously higher than control, but the frequency of HLA-A*11(P=0.031, OR=0.383, OR95%CI=0.158-0.930) was obviously lower than control (P<0.05). Conclusion The results showed that the alleles of HLA-A * 01, -A * 02, -B * 55, -DRB * 01, -DQB * 05 may associate with an antagonist effect in Eales disease, but HLA-A * 11 may be a protective gene of this disease.