CAJ | 학술논문

为探讨α辐射诱发体外细胞转化效应与hprt基因突变之间的相关关系。用体外培养的人胚肺细胞, 经0.25-5Gy α粒子照射后,各受照射剂量组细胞半固体琼脂培养集落形成能力明显增加,细胞获得了非锚着依赖性生长能力, 说明人胚肺细胞在α辐射作用下发生了转化细胞hprt基因突变检测。结果表明, 随着α粒子照射剂量的增加, hprt基因变异系数逐渐增加。细胞转化与hprt基因突变相关分析证实,两者之间存在显著的线性正相关关系。研究结果显示, α辐射的致癌效应与hprt基因突变密切相关。
위탐토α복사유발체외세포전화효응여hprt기인돌변지간적상관관계。용체외배양적인배폐세포, 경0.25-5Gy α입자조사후,각수조사제량조세포반고체경지배양집락형성능력명현증가,세포획득료비묘착의뢰성생장능력, 설명인배폐세포재α복사작용하발생료전화세포hprt기인돌변검측。결과표명, 수착α입자조사제량적증가, hprt기인변이계수축점증가。세포전화여hprt기인돌변상관분석증실,량자지간존재현저적선성정상관관계。연구결과현시, α복사적치암효응여hprt기인돌변밀절상관。
The purpose of this study is to investigate the relationship between cell transformation and hprt gene mutation induced by α irradiation in vitro. The results showed that the colony formation efficiency (CFE) of human embryo lung cells in soft agar increased after irradiation with 0.25-5Gy, which meant inducible cell transformation by α irradiation. Mutation frequency of hprt gene was found increased with the increase of the doses of α irradiation. Further analysis indicated the positive linear correlation between CFE and the variation coefficient of hprt gene. So, the carcinogenesis induced by α particles maybe is relative to hprt gene mutation.