CAJ | 학술논문

利用从菠菜(Spinacia oleracea L.)叶绿体分离、纯化出的缺失膜脂的细胞色素b6f蛋白复合体(Cyt b6f)制剂与从菠菜类囊体分离、纯化的膜脂进行体外重组,检测了不同膜脂对Cyt b6f催化电子传递活性的影响.结果表明:被检测的5种膜脂,即单半乳糖基甘油二酯(MGDG)、双半乳糖基甘油二酯(DGDG)、磷脂酰胆碱(PC)、磷脂酰甘油(PG)和硫代异鼠李糖基甘油二酯(SQDG)对Cyt b6f催化电子传递的活性均有明显的促进作用,但促进的程度各不相同,这可能与这些膜脂分子的带电性质密切相关.不带电荷的MGDG和DGDG及分子整体呈电中性的PC对促进Cyt b6f催化电子传递的活性非常有效,可分别使其活性提高89%、75%和77%;而带负电荷的PG和SQDG对活性的促进作用则相对较弱,仅可使其活性分别提高43%和26%.
이용종파채(Spinacia oleracea L.)협록체분리、순화출적결실막지적세포색소b6f단백복합체(Cyt b6f)제제여종파채류낭체분리、순화적막지진행체외중조,검측료불동막지대Cyt b6f최화전자전체활성적영향.결과표명:피검측적5충막지,즉단반유당기감유이지(MGDG)、쌍반유당기감유이지(DGDG)、린지선담감(PC)、린지선감유(PG)화류대이서리당기감유이지(SQDG)대Cyt b6f최화전자전체적활성균유명현적촉진작용,단촉진적정도각불상동,저가능여저사막지분자적대전성질밀절상관.불대전하적MGDG화DGDG급분자정체정전중성적PC대촉진Cyt b6f최화전자전체적활성비상유효,가분별사기활성제고89%、75%화77%;이대부전하적PG화SQDG대활성적촉진작용칙상대교약,부가사기활성분별제고43%화26%.
A lipid-depleted cytochrome b6f (Cyt b6f) preparation was obtained from spinach (Spinacia oler-acea L.) chloroplasts. Upon reconstitution of this preparation with the membrane lipids purified from spinachthylakoid, the effects of different membrane lipids on the electron transfer activity were studied. The resultsshow that the electron transfer activity of Cyt b6f is obviously stimulated to different extents, respectively, bymonogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), phosphatidylcholine (PC), phos-phatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG), and that the extents of stimulation may beclosely related to the charge of the membrane lipids. The stimulation of non-charged lipids (MGDG, DGDG)and neutrally-charged lipid (PC) was high with a maximum enhancement of 89%, 75% and 77%, respec-tively; but the stimulation of two kinds of negatively-charged lipid (PG and SQDG) was relatively low with amaximum enhancement of 43 % and 26%, respectively.