中国肺癌杂志
中國肺癌雜誌
중국폐암잡지
CHINESE JOURNAL OF LUNG CANCER
2001年
2期
88-90
,共3页
赵坡%钟梅%宋欣%吕亚利%王殿军%顾峥%陈乐真
趙坡%鐘梅%宋訢%呂亞利%王殿軍%顧崢%陳樂真
조파%종매%송흔%려아리%왕전군%고쟁%진악진
肝素酶基因%肺肿瘤%转移
肝素酶基因%肺腫瘤%轉移
간소매기인%폐종류%전이
目的 探讨肝素酶基因表达与肺癌转移的关系。方法 应用肝素酶基因引物、逆转录聚合酶链反应技术（RT-PCR）检测肺巨细胞癌株和56例原发肺癌及正常肺组织中肝素酶基因表达。结果 低转移和高转移的肺巨细胞癌株均为肝素酶基因表达阳性；已有淋巴结转移的肺癌阳性率为80％（20／25），而无淋巴结转移的肺癌阳性率为6.5％（2／31），两组间差异有显著性（P＜0.001）。提示伴有淋巴结转移的病例肝素酶基因表达阳性率明显高于不伴转移者。肝素酶基因表达多见于鳞癌（P＜0.05）和低分化癌(P＜0.01)，亦提示肝素酶基因表达可能与组织学分型及分化程度有关。结论 初步结果显示肝素酶基因表达可能为肺癌细胞获得转移活性的可靠标志之一，其检测可用于辅助判断肺癌患者预后。
目的 探討肝素酶基因錶達與肺癌轉移的關繫。方法 應用肝素酶基因引物、逆轉錄聚閤酶鏈反應技術（RT-PCR）檢測肺巨細胞癌株和56例原髮肺癌及正常肺組織中肝素酶基因錶達。結果 低轉移和高轉移的肺巨細胞癌株均為肝素酶基因錶達暘性；已有淋巴結轉移的肺癌暘性率為80％（20／25），而無淋巴結轉移的肺癌暘性率為6.5％（2／31），兩組間差異有顯著性（P＜0.001）。提示伴有淋巴結轉移的病例肝素酶基因錶達暘性率明顯高于不伴轉移者。肝素酶基因錶達多見于鱗癌（P＜0.05）和低分化癌(P＜0.01)，亦提示肝素酶基因錶達可能與組織學分型及分化程度有關。結論 初步結果顯示肝素酶基因錶達可能為肺癌細胞穫得轉移活性的可靠標誌之一，其檢測可用于輔助判斷肺癌患者預後。
목적 탐토간소매기인표체여폐암전이적관계。방법 응용간소매기인인물、역전록취합매련반응기술（RT-PCR）검측폐거세포암주화56례원발폐암급정상폐조직중간소매기인표체。결과 저전이화고전이적폐거세포암주균위간소매기인표체양성；이유림파결전이적폐암양성솔위80％（20／25），이무림파결전이적폐암양성솔위6.5％（2／31），량조간차이유현저성（P＜0.001）。제시반유림파결전이적병례간소매기인표체양성솔명현고우불반전이자。간소매기인표체다견우린암（P＜0.05）화저분화암(P＜0.01)，역제시간소매기인표체가능여조직학분형급분화정도유관。결론 초보결과현시간소매기인표체가능위폐암세포획득전이활성적가고표지지일，기검측가용우보조판단폐암환자예후。
Objective To investigate the relationship between the expressionof heparanase gene and the metastatic activity of lung cancer. Methods Using the primers of heparanase gene, the expression of heparanase gene was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in two giant cell lung cancer cell lines with different metastatic activity and 56 primary tumor tissues of lung cancer and normal lung tissues. Results Both cell lines and 80％ (20／25) of primary tumor tissues of lung cancer with lymph node metastasis showed positive expression of heparanase gene, whereas 6.5％ (2／31) primary tumor tissues of lung cancer without lymph node metastasis was positive and the normal lung tissues were all negative. The positive rate for expression of heparanase gene was significantly higher in the tumor tissues with lymph node metastasis than that without lymph node metastasis (P＜0.001), higher in squamous cell carcinoma (47.2％, 17／36) than that in adenocarcinoma (20％, 4／20)(P＜0.05), and remarkably higher in the poorly differentiated tumors (65％, 13／20) than that in the well-mediate differentiated tumors (22.2％, 8／36)(P＜0.01). Conclusion The preliminary results suggest that the expression of heparanase gene may be one of the reliable markers for the metastastic activity gained by the lung cancer cells and could be used clinically in predicting the prognosis of patients.
