CAJ | 학술논문

Objective To investigate the apoptosis of epididymis epithelium and the change of epididymis sialic acid following torsed/detorsed testes. Methods Twenty four adult male Sprague-Dawley rats were subjected to unilateral 720 testicular torsion with the duration of 2h and 4h, then repaired. The ischemic epididymis were collected for detecting the content of sialic acid by using spectrophotometry and the apoptosis with TUNEL technique.Results There were no statistically significant difference in the apoptosis of epididymis epithelium [ ( 9. 51 +2.78)% vs (6. 34 +1.98)% ] and the content of epididymis sialic acid(23. 3851 +9. 2199mg/mgprot vs 19. 36616. 3373mg/mgprot ) at 24h between following 2h-torsed/detorsed testes and those of sham group. There were statistically significant difference in the apopotosis of epididymis epithelium [ (46. 81 + 3. 55 )% vs (6. 34 ± 1.98 )% ] and the content of epididymis sialic acid ( 13. 7249 + 7. 8006mg/ mgprot vs 19. 3661 + 6. 3 373 mg/ mgprot ) at 24h between following 4h-torsed/detorsed testes and those of sham group ( P ＜ O. 05 ). Conclusion The results suggest that the sialic acid-secreting-function of epididymis remain normal at 24h following 2h-torsed/detorsed testes, while the apoptosis index of epididymis epithelium do not increase. The epididymis would be injured at 24h following 4htorsed/detorsed testes, while the apoptosis index increased.