CAJ | 학술논문

报道了在离体和田间条件下螺旋毛壳(C. spirale) ND35菌株对苹果树腐烂病的初步抑菌试验结果.PDA平板对峙培养的结果显示在毛壳ND35和苹果树腐烂病菌的两菌落间出现明显的抑菌带;毛壳ND35培养液粗提液比麦芽浸膏培养液的粗提液有更强的抑制病菌菌丝生长的作用.温室和田间生防试验表明用接种毛壳ND35的处理,其苹果树腐烂病发病率明显低于其它处理,包括直接种苹果树腐烂病菌和烫伤处理,及健康对照.再分离试验表明毛壳ND35可成功地在苹果树枝干部位定殖,而且固体麦麸菌剂的定殖率高于玉米粉液体菌剂的定殖率.但是玉米粉液体菌剂在田间的生防效率较好.
보도료재리체화전간조건하라선모각(C. spirale) ND35균주대평과수부란병적초보억균시험결과.PDA평판대치배양적결과현시재모각ND35화평과수부란병균적량균락간출현명현적억균대;모각ND35배양액조제액비맥아침고배양액적조제액유경강적억제병균균사생장적작용.온실화전간생방시험표명용접충모각ND35적처리,기평과수부란병발병솔명현저우기타처리,포괄직접충평과수부란병균화탕상처리,급건강대조.재분리시험표명모각ND35가성공지재평과수지간부위정식,이차고체맥부균제적정식솔고우옥미분액체균제적정식솔.단시옥미분액체균제재전간적생방효솔교호.
A new endophytic antagonistic fungus, Chaetomium spirale ND35 from Populus tomentosa, was reported. The bio-control trials of C. spirale ND35 against the Valsa Canker of apple were preliminarily investigated. The results of dual culture on PDA plate showed that C. spirale ND35 was capable of strong antagonism against Valsa ceratosperma, and for inhibiting the mycelial growth of V. ceratosperma,, the crude extract of liquid culture of corn steep powder broth was more effective than that one of malt extract broth (MEB). The results of bio-control in greenhouse and field indicated that the disease incidence of apple tree treated with C. spirale ND35 was lower significantly than that treated by other methods. The re-isolation experiment suggested that C. spirale ND35 could colonize in stems and branches of apple trees successfully, and the ND35 colonization rate of the treatment with solid wheat bran culture was higher than that of corn steep powder broth, but the field experiment result the control effect of liquid culture of C. spirale ND35 was better than that of solid culture.