农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
1期
29-31
,共3页
Seedless litchi%Young embryo%RNA extraction
A total RNA extraction method for young embryo of seedless litchi was introduced.CRAB,Phenol(saturated with water),chloroform,Guanidine isothiecyanate were used as main extraction reagents.Polyphenolic compounds were removed effectively by added PVP into the extraction buffer solution.RNA was purified intensively by phenol,chloroform extraction,and ethanol deposition after deposited by LiCl.Both the results of formaldehyde denatured agarose gel electrophoresis and ultraviolet spectrophotometer analysis showed high integrity and purity of RNA.So the quality of extracted RNA could meet the demand of most molecular biology experiments that require higher quality RNA.
