国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
1期
41-43
,共3页
凡瞿明%刘炳%张国元%程吉兵%卢晓岚%李薇
凡瞿明%劉炳%張國元%程吉兵%盧曉嵐%李薇
범구명%류병%장국원%정길병%로효람%리미
红斑狼疮,系统性%化学发光测定法%抗体
紅斑狼瘡,繫統性%化學髮光測定法%抗體
홍반랑창,계통성%화학발광측정법%항체
Lupus erythematosus，systemic%Chemiluminescent measurements%Antibodies
目的 探讨定量检测抗双链DNA(dsDNA)抗体对系统性红斑狼疮(SLE)疾病诊疗的价值.方法 将SLE疾病患者68 例分为SLE疾病活动组43例和SLE无明显疾病活动组25例;非SLE的其他自身免疫性疾病33例,健康对照组26例.采用化学发光法检测各组血清中抗dsDNA抗体,比较该抗体在4组研究对象血清中的差异,探讨抗dsDNA抗体定量检测在SLE疾病活动组与SLE无明显疾病活动组间的应用.结果 抗dsDNA抗体在SLE疾病活动组、SLE无明显疾病活动组、非SLE的其他自身免疫性疾病组和健康对照组间均值分别为126.41±99.62、36.57±65.10、10.87±22.37和6.82±11.09 IU/mL ;疾病活动组抗dsDNA抗体水平与其他组之间差异有统计学意义(P<0.01);该抗体在SLE中与血沉、疾病活动指数呈正相关,与补体C3、C4水平呈负相关.结论 抗dsDNA抗体定量检测对于SLE疾病活动性判断具有良好的应用价值,但不适合临床病程监测,即诊断的价值大于其监测价值.
目的 探討定量檢測抗雙鏈DNA(dsDNA)抗體對繫統性紅斑狼瘡(SLE)疾病診療的價值.方法 將SLE疾病患者68 例分為SLE疾病活動組43例和SLE無明顯疾病活動組25例;非SLE的其他自身免疫性疾病33例,健康對照組26例.採用化學髮光法檢測各組血清中抗dsDNA抗體,比較該抗體在4組研究對象血清中的差異,探討抗dsDNA抗體定量檢測在SLE疾病活動組與SLE無明顯疾病活動組間的應用.結果 抗dsDNA抗體在SLE疾病活動組、SLE無明顯疾病活動組、非SLE的其他自身免疫性疾病組和健康對照組間均值分彆為126.41±99.62、36.57±65.10、10.87±22.37和6.82±11.09 IU/mL ;疾病活動組抗dsDNA抗體水平與其他組之間差異有統計學意義(P<0.01);該抗體在SLE中與血沉、疾病活動指數呈正相關,與補體C3、C4水平呈負相關.結論 抗dsDNA抗體定量檢測對于SLE疾病活動性判斷具有良好的應用價值,但不適閤臨床病程鑑測,即診斷的價值大于其鑑測價值.
목적 탐토정량검측항쌍련DNA(dsDNA)항체대계통성홍반랑창(SLE)질병진료적개치.방법 장SLE질병환자68 례분위SLE질병활동조43례화SLE무명현질병활동조25례;비SLE적기타자신면역성질병33례,건강대조조26례.채용화학발광법검측각조혈청중항dsDNA항체,비교해항체재4조연구대상혈청중적차이,탐토항dsDNA항체정량검측재SLE질병활동조여SLE무명현질병활동조간적응용.결과 항dsDNA항체재SLE질병활동조、SLE무명현질병활동조、비SLE적기타자신면역성질병조화건강대조조간균치분별위126.41±99.62、36.57±65.10、10.87±22.37화6.82±11.09 IU/mL ;질병활동조항dsDNA항체수평여기타조지간차이유통계학의의(P<0.01);해항체재SLE중여혈침、질병활동지수정정상관,여보체C3、C4수평정부상관.결론 항dsDNA항체정량검측대우SLE질병활동성판단구유량호적응용개치,단불괄합림상병정감측,즉진단적개치대우기감측개치.
Objective To explore the clinical value of anti-dsDNA antibody quantitative detection in diagnosis and treatment of systemic lupus erythematosus (SLE).Methods Sixty-eight SLE patients were divided into the active group (n=43) and the stable group (n=25);besides, 33 patients suffered from other autoimmune disease and 26 healthy blood donors were enrolled in the study. Anti-dsDNA antibody in sera was detected by chemiluminescent immunoassay (CLIA). Then the quantity of anti-dsDNA antibody was compared in 4 groups and its applicable value between the active group and the stable group was analyzed.Results The levels of anti-dsDNA antibody in SLE active group, SLE stable group, other autoimmune disease group and healthy control group were 126.41±99.62，36.57±65.10, 10.87±22.3, 6.82±11.09 IU/mL respectively. There were significant differences between SLE active group and other groups (P<0.01). Anti-dsDNA antibody was positively correlated with ESR and SLE disease active index (DAI), while negatively correlated with C3 and C4.Conclusion The quantitive determination of anti-dsDNA antibody has great value in estimation of SLE activity, but not suitable for monitoring of the disease course. Therefore, its diagnostic value exceeds monitoring value.
