国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
3期
214-216
,共3页
稀释介质%生化项目%测定
稀釋介質%生化項目%測定
희석개질%생화항목%측정
Diluent%Biochemical parameters%Detection
目的 探讨生理盐水、蒸馏水及人灭活血清作稀释介质对部分生化项目测定的影响.方法 选取丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(GGT)、肌酸激酶(CK)、乳酸脱氢酶(LD)、а-羟丁酸脱氢酶(HBDH)、总胆红素(TBIL)、直接胆红素(DBIL)、胆汁酸(TBA)、肌酐(Cr)、尿酸(UA)、胆固醇(CHO)、糖(GLU)、尿素(BUN)等15个项目分析范围内不同浓度的标本各45份,分别用生理盐水、蒸馏水及人灭活血清稀释后用全自动生化分析仪进行测定,计算均值并进行统计学分析.结果 待测血清经生理盐水、蒸馏水或人灭活血清等不同介质稀释后,结果存在一定的差异.ALT、AST、GGT、DBIL、HBDH用10倍生理盐水、蒸馏水、人灭活血清稀释均可.ALP、TBA只能选人灭活血清,否则会使结果偏低.GLU、TBIL选择蒸馏水和人灭活血清稀释均可.BUN、CR、UA、CK、LDH、CHO选择生理盐水和人灭活血清更好,若选蒸馏水,除UA外其他结果均会降低.BUN不宜用蒸馏水稀释.用生理盐水稀释的项目5倍与10倍稀释的结果差异无统计学意义,可视具体情况选择.15个项目均可用人灭活血清作稀释介质.结论 为尽量减少稀释介质对测定的影响,上述生化项目应首选人灭活血清作稀释介质,若无备用人灭活血清可依上述结果进行相应稀释.
目的 探討生理鹽水、蒸餾水及人滅活血清作稀釋介質對部分生化項目測定的影響.方法 選取丙氨痠轉氨酶(ALT)、天門鼕氨痠轉氨酶(AST)、堿性燐痠酶(ALP)、γ-穀氨酰轉肽酶(GGT)、肌痠激酶(CK)、乳痠脫氫酶(LD)、а-羥丁痠脫氫酶(HBDH)、總膽紅素(TBIL)、直接膽紅素(DBIL)、膽汁痠(TBA)、肌酐(Cr)、尿痠(UA)、膽固醇(CHO)、糖(GLU)、尿素(BUN)等15箇項目分析範圍內不同濃度的標本各45份,分彆用生理鹽水、蒸餾水及人滅活血清稀釋後用全自動生化分析儀進行測定,計算均值併進行統計學分析.結果 待測血清經生理鹽水、蒸餾水或人滅活血清等不同介質稀釋後,結果存在一定的差異.ALT、AST、GGT、DBIL、HBDH用10倍生理鹽水、蒸餾水、人滅活血清稀釋均可.ALP、TBA隻能選人滅活血清,否則會使結果偏低.GLU、TBIL選擇蒸餾水和人滅活血清稀釋均可.BUN、CR、UA、CK、LDH、CHO選擇生理鹽水和人滅活血清更好,若選蒸餾水,除UA外其他結果均會降低.BUN不宜用蒸餾水稀釋.用生理鹽水稀釋的項目5倍與10倍稀釋的結果差異無統計學意義,可視具體情況選擇.15箇項目均可用人滅活血清作稀釋介質.結論 為儘量減少稀釋介質對測定的影響,上述生化項目應首選人滅活血清作稀釋介質,若無備用人滅活血清可依上述結果進行相應稀釋.
목적 탐토생리염수、증류수급인멸활혈청작희석개질대부분생화항목측정적영향.방법 선취병안산전안매(ALT)、천문동안산전안매(AST)、감성린산매(ALP)、γ-곡안선전태매(GGT)、기산격매(CK)、유산탈경매(LD)、а-간정산탈경매(HBDH)、총담홍소(TBIL)、직접담홍소(DBIL)、담즙산(TBA)、기항(Cr)、뇨산(UA)、담고순(CHO)、당(GLU)、뇨소(BUN)등15개항목분석범위내불동농도적표본각45빈,분별용생리염수、증류수급인멸활혈청희석후용전자동생화분석의진행측정,계산균치병진행통계학분석.결과 대측혈청경생리염수、증류수혹인멸활혈청등불동개질희석후,결과존재일정적차이.ALT、AST、GGT、DBIL、HBDH용10배생리염수、증류수、인멸활혈청희석균가.ALP、TBA지능선인멸활혈청,부칙회사결과편저.GLU、TBIL선택증류수화인멸활혈청희석균가.BUN、CR、UA、CK、LDH、CHO선택생리염수화인멸활혈청경호,약선증류수,제UA외기타결과균회강저.BUN불의용증류수희석.용생리염수희석적항목5배여10배희석적결과차이무통계학의의,가시구체정황선택.15개항목균가용인멸활혈청작희석개질.결론 위진량감소희석개질대측정적영향,상술생화항목응수선인멸활혈청작희석개질,약무비용인멸활혈청가의상술결과진행상응희석.
Objective To investigate the influence of different diluents(physiologic saline, dis-tilled water and human inactivated serum) on measurement of 15 items of biochemical parameters.Methods Fifteen items of biochemical parameters [alanine transaminase(ALT), aspartic transaminase (AST), alkaline phosphatase( ALP), gamma glutamyl transpeptidase ( GGT), creatine kinase ( CK),lactate dehydrogenase(LD), hydroxybutyrate dehydrogenase(HBDH), total bilirubin(TBIL), direct bilirubin(DBIL), total bile acid(TBA), ereatinine(Cr), uric acid(UA), eholesterol(CHO), glucose (GLU), and blood urea nitrogen(BUN)] were chosen. For each parameter, 45 serum samples with different eoncentrations of the parameter were collected. After diluted with different diluents(physio-logic saline, distilled water or human inactivated serum), the serum samples were detected by applying the fully automated biochemical analyzer. The mean value was calculated and statistical analysis was performed. Results There were some differences of detection results when the specimens were diluted with different diluents. ALT, AST, GGT, DBIL, and HBDH serum samples could be diluted by 10 times with physiologic saline, distilled water or human inactivated serums ALP and TBA serum sam-ples could only be diluted with inactivated serum, otherwise its result would be lower; GLU, TBIL samples could be diluted with distilled water and inactivated serums for BUN, CR, UA, CK, LDH,and CHO samples, physiologic saline or human inactivated serum might be optimal; if distilled water was chosen, the results of other parameters tented to decline except UA. It was BUN was improper to dilute the BUN samples with distilled water. In addition, there was no significant difference between the items diluted by 5 times and 10 times with physiologic saline. All the 15 items could be diluted with inactivated serum. Conclusion The inactivated serum should be the first choice of diluents to e-nure the accurate results of biochemical parameters. If the prepared inactivated serum is absent, we may choose other diluents according to the above-mentioned results.
