科技通报
科技通報
과기통보
BULLETIN OF SCIENCE AND TECHNOLOGY
2009年
4期
465-468,540
,共5页
杜黎明%胡天华%包崇来%朱琴妹%胡海娇%毛伟海
杜黎明%鬍天華%包崇來%硃琴妹%鬍海嬌%毛偉海
두려명%호천화%포숭래%주금매%호해교%모위해
不结球白菜%自交不亲和性%S基因型
不結毬白菜%自交不親和性%S基因型
불결구백채%자교불친화성%S기인형
non-heading Chinese cabbage%self-incompatibility%S-allele
为给不结球白菜的品种配置和遗传育种提供理论基础,利用特异性PCR扩增、DNA测序和生物信息学方法鉴定了7个自交系的S基因型,PCR扩增结果表明,51-14、375-124、711-114、884-214等4个自交系的基因型为Class-Ⅰ SLG和SRK,表现为自交强不亲和.523-112和544-312的基因型为Class-ⅡSLG类型.表现为自交若不亲和,和田间试验结果一致.测序结果blast分析表明844-214 Class-Ⅰ型和Brassica rapa SLG56的相似性为99%,51-14的SRK PCR与SRK56的同源性达到100%.
為給不結毬白菜的品種配置和遺傳育種提供理論基礎,利用特異性PCR擴增、DNA測序和生物信息學方法鑒定瞭7箇自交繫的S基因型,PCR擴增結果錶明,51-14、375-124、711-114、884-214等4箇自交繫的基因型為Class-Ⅰ SLG和SRK,錶現為自交彊不親和.523-112和544-312的基因型為Class-ⅡSLG類型.錶現為自交若不親和,和田間試驗結果一緻.測序結果blast分析錶明844-214 Class-Ⅰ型和Brassica rapa SLG56的相似性為99%,51-14的SRK PCR與SRK56的同源性達到100%.
위급불결구백채적품충배치화유전육충제공이론기출,이용특이성PCR확증、DNA측서화생물신식학방법감정료7개자교계적S기인형,PCR확증결과표명,51-14、375-124、711-114、884-214등4개자교계적기인형위Class-Ⅰ SLG화SRK,표현위자교강불친화.523-112화544-312적기인형위Class-ⅡSLG류형.표현위자교약불친화,화전간시험결과일치.측서결과blast분석표명844-214 Class-Ⅰ형화Brassica rapa SLG56적상사성위99%,51-14적SRK PCR여SRK56적동원성체도100%.
In order to provide a theoretical basis for the genetic breeding of non-heading Chinese cabbage,their Sgenotypes were identified by PCR amplification,DNA sequencing and bio-informatic analysis. PCR amplification shows that fragment was generated in 51-14、375-124、711-114,884-214 by primer Class-Ⅰ SLG and SRK. Fragment of about 1000 bp was generated in 523-112 and 544-312.DNA sequence bio-informatic analysis and BLA ST reveal that 844-214 similarity with Brassica rapa SRK56.
