CAJ | 학술논문

目的探讨Sysmex XE-2100血细胞分析仪白细胞分类的复检率,进一步完善临床检验科的标准操作程序(SOP)文件.方法使用Sysmex XE-2100血细胞分析仪随机检测537例非血液科患者的外周血标本同时做显微镜检查(包括细胞形态观察和白细胞分类计数分类),按照我国Sysmex XE-2100血细胞分析复检标准制定协作组制定的白细胞复检规则和显微镜检查阳性规则进行评估,计算出涂片复检率、真阳性、假阳性、真阴性、假阴性.结果根据Sysmex XE-2100血细胞分析复检标准制定协作组的23条复检规则和涂片阳性规则对检测结果进行统计分析,复检率为15.27%(82/537),真阳性率8.57%(46/537),假阳性率6.70%(36/537),真阴性率82.86%(445/537),假阴性率1.86%(10/537).结论 Sysmex XE-2100血细胞分析仪白细胞复检规则能有效提高检测效率,防止漏检和误检,可纳入本科室的SOP文件中.
목적 탐토Sysmex XE-2100혈세포분석의백세포분류적복검솔,진일보완선림상검험과적표준조작정서(SOP)문건.방법 사용Sysmex XE-2100혈세포분석의수궤검측537례비혈액과환자적외주혈표본동시주현미경검사(포괄세포형태관찰화백세포분류계수분류),안조아국Sysmex XE-2100혈세포분석복검표준제정협작조제정적백세포복검규칙화현미경검사양성규칙진행평고,계산출도편복검솔、진양성、가양성、진음성、가음성.결과 근거Sysmex XE-2100혈세포분석복검표준제정협작조적23조복검규칙화도편양성규칙대검측결과진행통계분석,복검솔위15.27%(82/537),진양성솔8.57%(46/537),가양성솔6.70%(36/537),진음성솔82.86%(445/537),가음성솔1.86%(10/537).결론 Sysmex XE-2100혈세포분석의백세포복검규칙능유효제고검측효솔,방지루검화오검,가납입본과실적SOP문건중.
Objective To explore the review rate of WBC differential analysis by Sysmex XE-2100 hematology analyzer and to perfect SOP files of the clinical laboratory.Methods On the basis of Hematology review criteria which was suggested by cooperation Group of formulation review criteria for automated CBC and WBC differential analysis by Sysmex XE-2100 hematology analyzer,537patients samples were randomly detected with automated hematology analyzer .Meanwhile, two blood smears were reviewed including observation of cell morphology and manual WBC differential analysis.Afterwards, we calculate the review rate,the true positive (TP)rate,the true negative(TN) rate,the false positive(FP) rate as well as the false negative(FN) rate.Results According to the 23 items of Sysmex XE-2100 slide review rule proposed by the group, the TP, FP, TN, FN were 8.57%, 6.7% 82. 86% and 1.86% respectively. And the Review Rate was 15.27%.Conclusion Review Criteria for WBC differential analysis by Sysmex XE-2100 hematology analyzer meets the needs of international consensus group .It can enhance work efficiency and reduce unnecessary labor work .It is available to obtain and could be added to the SOP files.