CAJ | 학술논문

目的观察银杏叶提取物对肺纤维化模型大鼠肺组织中结缔组织生长因子(CTGF)表达的影响.方法 96只健康Wistar大鼠随机分为正常对照组(A组)、模型组(B组)、强的松组(C组)和银杏叶提取物干预组(D组),每组各24只,除A组外,其余各组采用博莱霉素复制大鼠肺纤维化模型,造模第2天起,D组用银杏叶提取物灌胃,C组用强的松混悬液灌胃,A、B组用生理盐水灌胃.每组分别于7,14和28 d随机处死8只大鼠,制备肺组织切片,用免疫组化SABC法分别检测各时间点各组大鼠肺组织中的CTGF的表达.结果与A组比较,各时间点B、C、D组CTGF表达均显著增高(P＜0.01).与B组比较,各时间点,C、D各组CTGF表达均低于B组(P＜0.01或P＜0.05).结论 CTGF可能在肺泡炎、肺纤维化的发生发展过程中起着重要的作用,其表达程度与肺纤维化的程度密切相关;银杏叶提取物可能通过抑制CTGF的表达对肺组织起着修复及保护作用.
목적 관찰은행협제취물대폐섬유화모형대서폐조직중결체조직생장인자(CTGF)표체적영향.방법 96지건강Wistar대서수궤분위정상대조조(A조)、모형조(B조)、강적송조(C조)화은행협제취물간예조(D조),매조각24지,제A조외,기여각조채용박래매소복제대서폐섬유화모형,조모제2천기,D조용은행협제취물관위,C조용강적송혼현액관위,A、B조용생리염수관위.매조분별우7,14화28 d수궤처사8지대서,제비폐조직절편,용면역조화SABC법분별검측각시간점각조대서폐조직중적CTGF적표체.결과 여A조비교,각시간점B、C、D조CTGF표체균현저증고(P＜0.01).여B조비교,각시간점,C、D각조CTGF표체균저우B조(P＜0.01혹P＜0.05).결론 CTGF가능재폐포염、폐섬유화적발생발전과정중기착중요적작용,기표체정도여폐섬유화적정도밀절상관;은행협제취물가능통과억제CTGF적표체대폐조직기착수복급보호작용.
Objective To study the effect of ginkgo biloba extract on CTGF in experimental pulmonary fibrosis of rats.Methods 96 healthy Wistar rats,weighing from 180～220gram,were randomly divided into four groups(n=24,each):normal control group(group A),model group(group B),prednisone group(group C),Chinese drugs (group D).The rat model was induced by blyomicin and respective medicine was given to each group.Every group was sub-divided into three groups (n=8) which was killed on the 7th,14th and 28th day respectively,and the expression of CTGF in lung of the rats was detected.Results The expression of CTGF in the lung tissue in group C.D in the each phase was lower than that in group B respectively (P＜0.05or 0.01)Conclusion Ginkgo biloba extract can decrease the expression of CTGF in lung tissue.