CAJ | 학술논문

为了开发新型微生物农药用于家蚕病害防治,研究了桑树内生拮抗细菌洋葱伯克霍尔德氏菌(Burkholderia cepacia)Lu10-1菌株对家蚕白僵病的防治与抑菌效果.防治试验表明,Lu10-1菌株发酵上清液对家蚕白僵病的预防及治疗有效率分别达到55.88%、34.00%,且对家蚕生理及茧质无不良影响.体外抑菌试验表明,Lu10-1菌株的抗菌粗提物对白僵菌有较强的拮抗作用,抑菌圈直径为17.00 mm;抗菌粗提物还能够抑制白僵菌的生长及孢子萌发,当其作用于白僵菌孢子后,孢子出现聚集、内容物分布不均、变形、空腔及胞壁断裂等异常,作用于菌丝后,菌丝出现变粗、扭曲、皱褶、膨大、内容物流出、消融等现象;抑菌率随着抗菌粗提物浓度的增加而不断增大.初步认为Lu10-1菌株可以用于家蚕白僵病的防治.
위료개발신형미생물농약용우가잠병해방치,연구료상수내생길항세균양총백극곽이덕씨균(Burkholderia cepacia)Lu10-1균주대가잠백강병적방치여억균효과.방치시험표명,Lu10-1균주발효상청액대가잠백강병적예방급치료유효솔분별체도55.88%、34.00%,차대가잠생리급충질무불량영향.체외억균시험표명,Lu10-1균주적항균조제물대백강균유교강적길항작용,억균권직경위17.00 mm;항균조제물환능구억제백강균적생장급포자맹발,당기작용우백강균포자후,포자출현취집、내용물분포불균、변형、공강급포벽단렬등이상,작용우균사후,균사출현변조、뉴곡、추습、팽대、내용물류출、소융등현상;억균솔수착항균조제물농도적증가이불단증대.초보인위Lu10-1균주가이용우가잠백강병적방치.
For developing a new microbial pesticide to control silkworm diseases, the control effect and fungistasis of the antagonistic bacterium Burkholderia cepacia Lu10-1, isolated from mulberry, on silkworm muscardine were investigated. The results of control tests indicated that the supernatant of Lu10-1 zymotic fluid achieved 55.88% control efficiency and 34.00% prophylactic effect on silkworm white muscardine respectively, while no harmful effect was observed on the physiological functions and cocoon quality of the silkworm. The results of inhibitory tests indicated that the antibacterial crude extract of Lu10-1 had strong antagonistic activity against Beauveria bassiana. Its diameter of inhibition zone reached 17.00 mm. The antibacterial crude extract could also inhibit growth and spore germination of Beauveria bassiana. After being treated with the antibacterial crude extract, the spores of Beauveria bassiana became collective and had such abnormalities as uneven distribution of cellular contents, deformation of spores, disappear of cellular contents and disrupted cell wall;and the mycelia of Beauveria bassiana became thicker, retorted, corrugated, and inflated with inner substance outflowed and ablation of the cells. The inhibition efficiency increased with the concentration of antibacterial crude extract. It is preliminarily concluded that the Lu10-1 strain can be used to control silkworm white muscardine.