CAJ | 학술논문

目的检测基底/肌上皮标记物在三联阴性乳腺癌的表达情况,并分析基底细胞样癌的形态学特征.方法收集69例三联阴性乳腺癌存档蜡块,用单克隆抗体EGFR、CK5/6、CK14、CK17、SMA和CD10进行免疫组化MaxVision~(TM)法染色,并将其分为基底细胞样型(BLs)(表达任意一种标记物)和Null型(均阴性).重点观察两者的形态学差异.结果 BLs型48例(69.6%),Null型21例(30.4%).具有统计学差异的形态学特征包括巢片状结构、推挤或部分推挤性边界、组织学分级高、核分级高和高核分裂指数,其中BLs型中42例呈巢片状结构(87.5%),26例呈推挤或部分推挤性边界(54.2%).结论基底细胞样型与Null型乳腺癌具有明显的形态学差异,最具诊断价值的形态特点是巢片状结构、组织分级高、中到大量淋巴细胞浸润、推挤或部分推挤性边界和高核分裂指数.最具应用价值的标记物是CK5/6.
목적 검측기저/기상피표기물재삼련음성유선암적표체정황,병분석기저세포양암적형태학특정.방법 수집69례삼련음성유선암존당사괴,용단극륭항체EGFR、CK5/6、CK14、CK17、SMA화CD10진행면역조화MaxVision~(TM)법염색,병장기분위기저세포양형(BLs)(표체임의일충표기물)화Null형(균음성).중점관찰량자적형태학차이.결과 BLs형48례(69.6%),Null형21례(30.4%).구유통계학차이적형태학특정포괄소편상결구、추제혹부분추제성변계、조직학분급고、핵분급고화고핵분렬지수,기중BLs형중42례정소편상결구(87.5%),26례정추제혹부분추제성변계(54.2%).결론 기저세포양형여Null형유선암구유명현적형태학차이,최구진단개치적형태특점시소편상결구、조직분급고、중도대량림파세포침윤、추제혹부분추제성변계화고핵분렬지수.최구응용개치적표기물시CK5/6.
Objective To detect the expression of basal/myoepithelial markers in triple negative breast carcinoma, and to summarize the morphological features of basal-like subtype of invasive breast carcinoma. Methods Immunohistochemistry was performed in 69 cases of triple negative breast carcinomas, with CD 5/6, CK14, CK17, SMA and CD10 monoclonal antibodies. Two subtypes were classified according to immunophenotypes: basal-like subtype (positive to anyone or more antibodies) and null subtype (negative for all). The emphasis was put on morphological difference between the two subtypes. Results The number of basal-like subtype was 48 (69.6%), and null subtype 21 (30.4%). Compared to null stubtype, hasal-like subtype had significantly more nest or sheet arrangement, high histological grade, pushing or partly pushing border of invasion, high nuclear grade (P = 0.002) and high mitotic count among of them, in which the most sensitive feature was nest or sheet arrangement (42/ 48), and the most specific feature was pushing or partly pushing border of invasion (26/31). Conclusion The helpful morpthologic features include nest or sheet arrangement, high histological grade, pushing or partly pushing border of invasion, high nuclear grade and high mitotic count. The most useful marker is CK 5/6.