CAJ | 학술논문

目的:观察结直肠癌组织中k-ras基因突变情况,探讨k-ras基因突变与结直肠癌生物学行为的关系.方法:采用实时荧光定量PCR法检测123例结直肠癌组织中k-ras基因1号外显子12、13密码子突变情况,结合其临床病理资料分析.结果:123例结直肠癌组织中k-ras基因突变者53例(40.8%),其中12密码子突变42例(34.1%),13密码子突变者11例(8.9%).基因突变率与肿瘤大小、肿瘤侵润深度、分化程度无明显相关性,与淋巴结转移、肝脏转移及TNM分期有相关性(P<0.05).淋巴结转移多者k-ras基因突变率高,有肝脏转移者基因突变率高,TNM分期越晚基因突变率越高.结论:K-ras基因突变可能在结直肠癌的发生、发展中起重要作用,而且与淋巴结转移和肝脏转移有密切相关,可作为判断结直肠癌恶性程度的一个分子生物学指标.
목적:관찰결직장암조직중k-ras기인돌변정황,탐토k-ras기인돌변여결직장암생물학행위적관계.방법:채용실시형광정량PCR법검측123례결직장암조직중k-ras기인1호외현자12、13밀마자돌변정황,결합기림상병리자료분석.결과:123례결직장암조직중k-ras기인돌변자53례(40.8%),기중12밀마자돌변42례(34.1%),13밀마자돌변자11례(8.9%).기인돌변솔여종류대소、종류침윤심도、분화정도무명현상관성,여림파결전이、간장전이급TNM분기유상관성(P<0.05).림파결전이다자k-ras기인돌변솔고,유간장전이자기인돌변솔고,TNM분기월만기인돌변솔월고.결론:K-ras기인돌변가능재결직장암적발생、발전중기중요작용,이차여림파결전이화간장전이유밀절상관,가작위판단결직장암악성정도적일개분자생물학지표.
AIM: To investigate mutations of oncogene k-ras in colorectal cancer tissues and the relationship between mutations of k - ras and biological behavior of colorectal carcinoma. METHODS:The specimens of 123 patients with colorectal cancer were collected. Real - time fluorescence quantitative PCR were performed to detect k-ras mutations at codon 12 and codon 13 of exon 1, and the results were analyzed with the corresponding clinical pathological data. RESULTS: Among 123 colorectal cancer cases, point mutations were detected in 53 cases (40.8% ) , point mutations at codon 12 were found in 42 (34.1 % ) cases, and 11(8.9% ) cases at codon 13.No closely relationship between mutations of k-ras and tumor size, location, invasive depth and differentiation extent was observed. The rate of k-ras mutation in the cases with more invaded lymph nodes was higher than that in the cases without invaded lymph nodes ( P < 0.05 ) , and the rate of k-ras gene mutation in the cases with hepatic metastases was higher than that in no hepatic metastases (P <0.05). The rate of k - ras gene mutation was higher in TNM staging Ⅲ/Ⅳ than that in Ⅰ/Ⅱ( P < 0.05 ). CONCLUSION: Mutation of oncogene k-ras plays an important role in the carcinogenesis and development of colorectal cancer, and it is closely associated with invaded lymph notes and hepatic metastases, suggesting that mutation of k- ras indicates a poor prognosis.