CAJ | 학술논문

根据水稻β-1,4-糖苷酶(korrigan)基因的保守区序列设计引物,以毛竹cDNA为模板,采用PCR方法,成功扩增出1个含有完整阅读框架的cDNA序列,长度为2 191 bp,共编码617个氨基酸,将其命名为PeKOR基因.其氨基酸序列分析的结果表明,PeKOR与其他β-1,4-糖苷酶有较高的同源性,同水稻序列相似性高达91%,且其序列具有典型的Glycosyl hydrolase 9 super family结构域,推测此PeKOR为毛竹β-1,4-糖苷酶基因.在竹笋中采用半定量方法研究该基因的表达情况,结果表明该基因在高温条件下表达量较低温条件下明显升高.
근거수도β-1,4-당감매(korrigan)기인적보수구서렬설계인물,이모죽cDNA위모판,채용PCR방법,성공확증출1개함유완정열독광가적cDNA서렬,장도위2 191 bp,공편마617개안기산,장기명명위PeKOR기인.기안기산서렬분석적결과표명,PeKOR여기타β-1,4-당감매유교고적동원성,동수도서렬상사성고체91%,차기서렬구유전형적Glycosyl hydrolase 9 super family결구역,추측차PeKOR위모죽β-1,4-당감매기인.재죽순중채용반정량방법연구해기인적표체정황,결과표명해기인재고온조건하표체량교저온조건하명현승고.
A beta-1,4-glycosidase protein gene from phyllostachys edulis,named as PeKOR,was cloned through PCR using primers designed according to the beta-1,4-glycosidase protein genes conserved region of other plant.The coding region of the genomic clone of PeKOR is continuous.The cDNA of PeKORcontains an open reading frame of 2 191 bp and codes for a protein of 617 aa.The database search using the amino acid sequence as query showed high homology to several beta-1,4-glycosidase proteins,especially the PeKOR sequence showed the maximum homology(91% identity)to Oryza sativa subspecies beta-1,4-glycosidase protein gene),and it has a typically conserved domain of Glycosyl hydrolase 9 super family.Therefore we predicted that the PeKOR could be an beta-1,4-glycosidase genein bamboo.The result of semiquantitative RT-PCR in bamboo shoots showed that expression of PeKOR gene was regulated by temperature,and the expression level on high temperature is larger than the one on low temperature.