药学学报
藥學學報
약학학보
ACTA PHARMACEUTICA SINICA
2007年
11期
1183-1188
,共6页
马宁%刘文英%李焕德%蒋新宇%张毕奎%朱荣华%王峰%谢悦良%周孝钱%吴雪%向大雄
馬寧%劉文英%李煥德%蔣新宇%張畢奎%硃榮華%王峰%謝悅良%週孝錢%吳雪%嚮大雄
마저%류문영%리환덕%장신우%장필규%주영화%왕봉%사열량%주효전%오설%향대웅
(E)-3,5,4'-三甲氧基-1,2-二苯乙烯%高效液相色谱法%白藜芦醇
(E)-3,5,4'-三甲氧基-1,2-二苯乙烯%高效液相色譜法%白藜蘆醇
(E)-3,5,4'-삼갑양기-1,2-이분을희%고효액상색보법%백려호순
(E) -3,5,4'-trimethoxystilbene%HPLC%resveratrol
建立大鼠血浆与组织中白藜芦醇衍生物(BTM-0512)高效液相色谱测定方法,用于研究BTM-0512在大鼠体内的分布.色谱柱:BDS Hypersil C18(250 mm ×4.6 mm ID,5 μm),流动相:乙腈-水(55:45),流速:1 mL·min-1,检测波长:319 nm,己烯雌酚作内标.含药血浆与组织匀浆液用乙腈直接沉淀蛋白后,进样分析.本法线性范围为血浆:0.01～10.0 μg·mL-1,组织样品:0.04～40 μg·g-1,r2均大于0.996(n=7),方法的准确度平均值在95.3%～100.1%;提取回收率在95.9%～100.9%;日内、日间RSD＜5.1%.BTM-0512在血浆中含量较低,在肝脏中分布最多,在脂肪中有蓄积现象.本法灵敏度高,操作简便、准确,可用于大鼠血浆及组织中BTM-0512的测定及分布研究.
建立大鼠血漿與組織中白藜蘆醇衍生物(BTM-0512)高效液相色譜測定方法,用于研究BTM-0512在大鼠體內的分佈.色譜柱:BDS Hypersil C18(250 mm ×4.6 mm ID,5 μm),流動相:乙腈-水(55:45),流速:1 mL·min-1,檢測波長:319 nm,己烯雌酚作內標.含藥血漿與組織勻漿液用乙腈直接沉澱蛋白後,進樣分析.本法線性範圍為血漿:0.01～10.0 μg·mL-1,組織樣品:0.04～40 μg·g-1,r2均大于0.996(n=7),方法的準確度平均值在95.3%～100.1%;提取迴收率在95.9%～100.9%;日內、日間RSD＜5.1%.BTM-0512在血漿中含量較低,在肝髒中分佈最多,在脂肪中有蓄積現象.本法靈敏度高,操作簡便、準確,可用于大鼠血漿及組織中BTM-0512的測定及分佈研究.
건립대서혈장여조직중백려호순연생물(BTM-0512)고효액상색보측정방법,용우연구BTM-0512재대서체내적분포.색보주:BDS Hypersil C18(250 mm ×4.6 mm ID,5 μm),류동상:을정-수(55:45),류속:1 mL·min-1,검측파장:319 nm,기희자분작내표.함약혈장여조직균장액용을정직접침정단백후,진양분석.본법선성범위위혈장:0.01～10.0 μg·mL-1,조직양품:0.04～40 μg·g-1,r2균대우0.996(n=7),방법적준학도평균치재95.3%～100.1%;제취회수솔재95.9%～100.9%;일내、일간RSD＜5.1%.BTM-0512재혈장중함량교저,재간장중분포최다,재지방중유축적현상.본법령민도고,조작간편、준학,가용우대서혈장급조직중BTM-0512적측정급분포연구.
This paper described a rapid and se nsitive HPLC method to analyze (E)-3, 5,4'-trimethoxystilbene (BTM-0512) in rat plasma and tissues. The analysis used a BDS Hypersil C18 analytical column (250 mm×4.6 mm ID, 5 μm) and acetonitrile / water as the mobile phase. The UV detection wavelength was 319 nm. Proteins were precipitated with acetonitrile and diethylstilbestrol as internal standard. The method was validated according to State Food and Drug Administration of China and ICH of Technical Requirements for Registration of Pharmaceuticals for Human Use Guidelines. The limit of interday precision values (%RSD) were in the range of 2.6% -5.1% and 2.4% -4.8%, respectively.Mean accuracy and absolute recoveries of BTM-0512 ranged from 95.3% - 100. 1% and 95.9% -100.9% for plasma and tissues, respectively. This method can be quite useful for BTM-0512 pharmacokinetic and tissue distribution studies, for purpose which multiple plasma and tissue samples can be analyzed quickly with high reproducibility.
