中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2011年
6期
633-637
,共5页
魏绍峰%张爱华%梁冰%黄晓欣
魏紹峰%張愛華%樑冰%黃曉訢
위소봉%장애화%량빙%황효흔
砷中毒%煤%多态现象,遗传%基因频率
砷中毒%煤%多態現象,遺傳%基因頻率
신중독%매%다태현상,유전%기인빈솔
Arsenic poisoning%Coal%Polymorphism,genetic%Gene frequency
目的 探讨核苷酸切除修复基因ERCCI、XPD、XPC不同基因型与燃煤污染型砷中毒发病风险的关系.方法 以贵州省兴仁县交乐村燃煤污染型砷中毒病区229例砷中毒患者作为病例组,以有相似生活习惯、无燃用高砷煤史的非砷暴露村大果朵村198名居民作为对照组,每人抽取外周静脉血约2 ml提取DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术进行ERCC1 C8092A 、XPD Lys751 Gln、XPD Asp312Asn、XPD Arg156Arg、XPC P(AT+/-)多态位点检测.结果 病例组ERCC1 C8092A位点CA/AA基因型分布频率[ CA:29.78％ (67/225)、AA:10.67％ (24/225)]显著高于对照组[CA:23.08％( 45/195)、AA:5.13％ (10/195),x2=8.116,P＜0.05]；其余各基因多态位点的基因型分布频率差异无统计学意义(x2值分别为5.649、4.394、0.865、1.490,P均＞0.05).携带ERCCI 8092CA+ AA、XPD Lys751Gln+ Gln751Gln 、XPD Asp312Asn+ Asn312Asn基因型个体分别较携带ERCC1 8092CC、XPD Lys751Lys、XPD Asp312Asp基因型个体发生砷中毒的风险升高1.780、1.681、1.790倍(95％CI分别为1.174～2.698、1.081～2.615和1.014～3.158,P均＜0.05)；单一的XPD 基因Arg156Arg位点、XPC基因P(AT+/-)位点对砷中毒的发病风险没有影响(P均＞0.05).结论 核苷酸切除修复基因ERCC1 C8092A、XPD Lys751 Gln和Asp312Asn位点的多态性与燃煤污染型砷中毒的发病风险有关.
目的 探討覈苷痠切除脩複基因ERCCI、XPD、XPC不同基因型與燃煤汙染型砷中毒髮病風險的關繫.方法 以貴州省興仁縣交樂村燃煤汙染型砷中毒病區229例砷中毒患者作為病例組,以有相似生活習慣、無燃用高砷煤史的非砷暴露村大果朵村198名居民作為對照組,每人抽取外週靜脈血約2 ml提取DNA,採用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)技術進行ERCC1 C8092A 、XPD Lys751 Gln、XPD Asp312Asn、XPD Arg156Arg、XPC P(AT+/-)多態位點檢測.結果 病例組ERCC1 C8092A位點CA/AA基因型分佈頻率[ CA:29.78％ (67/225)、AA:10.67％ (24/225)]顯著高于對照組[CA:23.08％( 45/195)、AA:5.13％ (10/195),x2=8.116,P＜0.05]；其餘各基因多態位點的基因型分佈頻率差異無統計學意義(x2值分彆為5.649、4.394、0.865、1.490,P均＞0.05).攜帶ERCCI 8092CA+ AA、XPD Lys751Gln+ Gln751Gln 、XPD Asp312Asn+ Asn312Asn基因型箇體分彆較攜帶ERCC1 8092CC、XPD Lys751Lys、XPD Asp312Asp基因型箇體髮生砷中毒的風險升高1.780、1.681、1.790倍(95％CI分彆為1.174～2.698、1.081～2.615和1.014～3.158,P均＜0.05)；單一的XPD 基因Arg156Arg位點、XPC基因P(AT+/-)位點對砷中毒的髮病風險沒有影響(P均＞0.05).結論 覈苷痠切除脩複基因ERCC1 C8092A、XPD Lys751 Gln和Asp312Asn位點的多態性與燃煤汙染型砷中毒的髮病風險有關.
목적 탐토핵감산절제수복기인ERCCI、XPD、XPC불동기인형여연매오염형신중독발병풍험적관계.방법 이귀주성흥인현교악촌연매오염형신중독병구229례신중독환자작위병례조,이유상사생활습관、무연용고신매사적비신폭로촌대과타촌198명거민작위대조조,매인추취외주정맥혈약2 ml제취DNA,채용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술진행ERCC1 C8092A 、XPD Lys751 Gln、XPD Asp312Asn、XPD Arg156Arg、XPC P(AT+/-)다태위점검측.결과 병례조ERCC1 C8092A위점CA/AA기인형분포빈솔[ CA:29.78％ (67/225)、AA:10.67％ (24/225)]현저고우대조조[CA:23.08％( 45/195)、AA:5.13％ (10/195),x2=8.116,P＜0.05]；기여각기인다태위점적기인형분포빈솔차이무통계학의의(x2치분별위5.649、4.394、0.865、1.490,P균＞0.05).휴대ERCCI 8092CA+ AA、XPD Lys751Gln+ Gln751Gln 、XPD Asp312Asn+ Asn312Asn기인형개체분별교휴대ERCC1 8092CC、XPD Lys751Lys、XPD Asp312Asp기인형개체발생신중독적풍험승고1.780、1.681、1.790배(95％CI분별위1.174～2.698、1.081～2.615화1.014～3.158,P균＜0.05)；단일적XPD 기인Arg156Arg위점、XPC기인P(AT+/-)위점대신중독적발병풍험몰유영향(P균＞0.05).결론 핵감산절제수복기인ERCC1 C8092A、XPD Lys751 Gln화Asp312Asn위점적다태성여연매오염형신중독적발병풍험유관.
Objective To investigate the relationship between genetic polymorphisms in excision repair cross-complementing 1 (ERCC 1 ),xeroderma pigmentosum group D (XPD),xeroderma pigmentosum group C (XPC) and the risk of arsenism caused by coal-burning.Methods Two hundred and twenty-nine patients with arsenism in the endemic area of Jiaole village Xingren county Guizhou province were selected into experimental group.One hundred and ninety-eight inhabitants who had similar living habits but did no burning coal with high arsenic in Dagnoduo village were selected into control group.Two milliliters vein blood samples were taken and analyzed with polymerase chain reaction-restriction frgment length polymorphism technique (PCR-RFLP) to measure the gene polymorphisms of ERCC1 C8092A,XPD Lys751Gln,XPD Asp312Asn,XPD Arg156Arg,and XPC P(AT +/-).Relationship between genotype and the risk of arsenism was also analyzed.Results The frequency of ERCC1 8092CA/AA geno-type in case group [ CA:29.78％ (67/225),AA:10.67％ (24/225) ] was significantly higher than that of control group[CA:23.08％(45/195),AA:5.13％(10/195),x2 =8.116,P ＜ 0.05].The frequency difference of other gene polymorphisms between case and control group was not statistically significant,respectively (x2 =5.649,4.394,0.865,1.490,all P ＞ 0.05).There were 1.780(95％CI:1.174 - 2.698),1.681(95％CI:1.081 - 2.615),and 1.790(95％CI:1.014 - 3.158)-fold increase in risk of arsenism for individuals carrying ERCC1 8092CA + AA,XPD Lys751Gln + Gln751Gln,and XPD Asp312Asn + Asn312Asn genotypes compared respectively with individuals canying ERCC1 8092CC,XPD Lys751Lys,and XPD Asp312Asp(all P ＜ 0.05).The sufferers only with XPD Arg156Arg or XPC P(AT +/-) didn't have higher risk of arsenism(all P ＞ 0.05).Conclusion The results of this study suggest that the gene polymorphisms of ERCC1 C8092A,XPD Lys751Gln,and Asp312Asn are related to the arsenism caused by coal-burning.
