CAJ | 학술논문

目的研究TGF-β1基因多态性在SARS-冠状病毒(SARS-CoV)感染及愈后肺间质纤维化中的作用.方法选取65例SARS康复人员、37例医护人员和66例健康人员,进行TGF-β1基因多态性与SARS-CoV易感性及与SARS愈后肺问质纤维化关系的分析.采用病例一病例研究设计,在SARS康复患者中分析TGF-β1基因多态性与SARS愈后肺间质纤维化的关系.采用聚合酶链反应-直接测序法(PCR-SBT)进行TGF-β1基因多态性检测,数据行t榆验和卡方检验.结果 TGF-β1基因+869和+915位点的基因多态性在SARS康复人群、医护人员和对照人群分布差异无统计学意义,未见该基因两位点的多态性连锁与SARS-CoV易感性有关.SARS愈后人群的肺间质纤维化改变与FGF-β1基因+869和+915位点的基因多态性无关,也未见该基因+869和+915位点的多态性连锁与肺间质纤维化相关.结论 TGF-β1基因+869和+915位点的基因多态性可能与SARS-CoV易感性无关;SARS康复人员的肺间质纤维化可能与TGF-β1+869和+915基因多态性无关.
목적 연구TGF-β1기인다태성재SARS-관상병독(SARS-CoV)감염급유후폐간질섬유화중적작용.방법 선취65례SARS강복인원、37례의호인원화66례건강인원,진행TGF-β1기인다태성여SARS-CoV역감성급여SARS유후폐문질섬유화관계적분석.채용병례일병례연구설계,재SARS강복환자중분석TGF-β1기인다태성여SARS유후폐간질섬유화적관계.채용취합매련반응-직접측서법(PCR-SBT)진행TGF-β1기인다태성검측,수거행t유험화잡방검험.결과 TGF-β1기인+869화+915위점적기인다태성재SARS강복인군、의호인원화대조인군분포차이무통계학의의,미견해기인량위점적다태성련쇄여SARS-CoV역감성유관.SARS유후인군적폐간질섬유화개변여FGF-β1기인+869화+915위점적기인다태성무관,야미견해기인+869화+915위점적다태성련쇄여폐간질섬유화상관.결론 TGF-β1기인+869화+915위점적기인다태성가능여SARS-CoV역감성무관;SARS강복인원적폐간질섬유화가능여TGF-β1+869화+915기인다태성무관.
Objective To investigate the roles of transforming growth factor(TGF)-β1 gene polymorphisms in severe acute respiratory syndrome-coronavirus(SARS-CoV)infection and the interstitial lung fibrosis after recovered.Methods Sixty-five recovered SARS patients,37 health care workers and 66 healthy controls were enrolled in this case-case study.The association between genetic polymorphisms of TG F-β1 and suscept ibility to SARS or interstitial lung changes after SARS reco,vered was carried out.Polymerase chain reaction-sequencing based typing(PCR-SBT)method was used to determine the polymorphisms of TGF-β1 gene at locus+869 and+915.Data were analyzed using t test and chi square test.Results There was no significant association of TGF+β1 gene polymorphisms at locus+869 and+915 in recovered SARS patients,health care workers and heahhy controls.And gene linkage of this two loci was not related with SARS-CoV susceptibility.Furthermore,no association between interstitial lung changes in recovered SARS palients and TGF-β1 gene polymorphisms or genetic linkage of this two loci.Conclusions It may not be related between TGFβ1 gene polymorphisms at locus+869 and+915 and SARS-CoV susceptibility.And interstitial lung changes in recovered SARS patients may not be influenced by TGF-β1 gene polymorphisms.