国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2011年
2期
78-81
,共4页
边学海%张广%张纯海%张大奇%周乐%孙辉
邊學海%張廣%張純海%張大奇%週樂%孫輝
변학해%장엄%장순해%장대기%주악%손휘
甲状腺乳头状癌%Shh%Gli1%免疫组织化学
甲狀腺乳頭狀癌%Shh%Gli1%免疫組織化學
갑상선유두상암%Shh%Gli1%면역조직화학
Papillary thyroid carcinoma%Shh%Gli1%Immunohistochemistry
目的 通过检测Sonic Hedgehog信号通路关键分子Shh和Gil1在甲状腺乳头状癌表达的情况,探讨其与甲状腺乳头状癌临床病理特征的关系及临床意义.方法 应用免疫组织化学方法检测142例甲状腺乳头状癌患者癌组织及其癌旁组织病理蜡块标本中Shh和Gli1的表达情况,分析其与临床病理特征的关系.结果 Shh主要表达于细胞膜与细胞质中,Gli1表达于细胞浆和细胞核内.甲状腺乳头状癌组织中Shh和Gil1阳性表达率分别为64.1%(91/142)和47.9%(68/142)明显高于癌旁组织的16.9%(24/142)和9.2%(13/142).Gil1的表达与患者年龄有关(P＜0.05).Shh和Gil1与肿瘤的TNM分期显著相关(P＜0.01).Shh和Gil1在肿瘤长径≥1 cm的表达阳性率分别为79.2%和60.4%,均高于肿瘤长径＜1 cm的表达阳性率,差异均具有统计学意义,Shh更为显著(P＜0.01).Shh和Gil1在有淋巴结转移的PTC组织中的表达阳性率分别为72.5%和65.2%,差异有统计学意义,Gill更为显著(P＜0.01).结论 Shh信号通路的主要配体Shh和Gil1在甲状腺乳头状癌中表达升高,并与肿瘤发生、分期及淋巴结转移有关,提示Shh信号通路的异常激活在甲状腺乳头状癌中发挥一定的作用,Shh和Gil1为甲状腺乳头状癌预后判断指标及潜在的治疗靶点.
目的 通過檢測Sonic Hedgehog信號通路關鍵分子Shh和Gil1在甲狀腺乳頭狀癌錶達的情況,探討其與甲狀腺乳頭狀癌臨床病理特徵的關繫及臨床意義.方法 應用免疫組織化學方法檢測142例甲狀腺乳頭狀癌患者癌組織及其癌徬組織病理蠟塊標本中Shh和Gli1的錶達情況,分析其與臨床病理特徵的關繫.結果 Shh主要錶達于細胞膜與細胞質中,Gli1錶達于細胞漿和細胞覈內.甲狀腺乳頭狀癌組織中Shh和Gil1暘性錶達率分彆為64.1%(91/142)和47.9%(68/142)明顯高于癌徬組織的16.9%(24/142)和9.2%(13/142).Gil1的錶達與患者年齡有關(P＜0.05).Shh和Gil1與腫瘤的TNM分期顯著相關(P＜0.01).Shh和Gil1在腫瘤長徑≥1 cm的錶達暘性率分彆為79.2%和60.4%,均高于腫瘤長徑＜1 cm的錶達暘性率,差異均具有統計學意義,Shh更為顯著(P＜0.01).Shh和Gil1在有淋巴結轉移的PTC組織中的錶達暘性率分彆為72.5%和65.2%,差異有統計學意義,Gill更為顯著(P＜0.01).結論 Shh信號通路的主要配體Shh和Gil1在甲狀腺乳頭狀癌中錶達升高,併與腫瘤髮生、分期及淋巴結轉移有關,提示Shh信號通路的異常激活在甲狀腺乳頭狀癌中髮揮一定的作用,Shh和Gil1為甲狀腺乳頭狀癌預後判斷指標及潛在的治療靶點.
목적 통과검측Sonic Hedgehog신호통로관건분자Shh화Gil1재갑상선유두상암표체적정황,탐토기여갑상선유두상암림상병리특정적관계급림상의의.방법 응용면역조직화학방법검측142례갑상선유두상암환자암조직급기암방조직병리사괴표본중Shh화Gli1적표체정황,분석기여림상병리특정적관계.결과 Shh주요표체우세포막여세포질중,Gli1표체우세포장화세포핵내.갑상선유두상암조직중Shh화Gil1양성표체솔분별위64.1%(91/142)화47.9%(68/142)명현고우암방조직적16.9%(24/142)화9.2%(13/142).Gil1적표체여환자년령유관(P＜0.05).Shh화Gil1여종류적TNM분기현저상관(P＜0.01).Shh화Gil1재종류장경≥1 cm적표체양성솔분별위79.2%화60.4%,균고우종류장경＜1 cm적표체양성솔,차이균구유통계학의의,Shh경위현저(P＜0.01).Shh화Gil1재유림파결전이적PTC조직중적표체양성솔분별위72.5%화65.2%,차이유통계학의의,Gill경위현저(P＜0.01).결론 Shh신호통로적주요배체Shh화Gil1재갑상선유두상암중표체승고,병여종류발생、분기급림파결전이유관,제시Shh신호통로적이상격활재갑상선유두상암중발휘일정적작용,Shh화Gil1위갑상선유두상암예후판단지표급잠재적치료파점.
Objective To define the expression of Shh/Gli1,the key elements of Hedgehog signaling pathway in papillary thyroid carcinoma(PTC) and to explore the relationship between the expression of Shh/Gli1 and clinical significance.Methods The expression of Shh and Gli1 was examined in 142 cases of PTC tissues and adjacent tumor thyroid tissues as control by immunohistochemistry.The relationship between the expression of Shh/Gli1 and clinical characteristics of PTC patients was analyzed.Results The positive rate of the cytoplasm Shh expression and the nuclear Gli1 expression was 64.1% and 47.9% ,respectively.Significant difference was found between normal thyroid tissues and PTC.The research showed that the expression of Shh/Gli1 was related to the tumor size,clinical stages and lymph node metastasis,Shh was more significantly related to the tumor size(P ＜0.01) and Gli1 was more significantly related to the lymph node metastasis (P ＜ 0.01).Conclusions Varying expression of the main ligand Shh and transcription factor Gli1 in Hedgehog signaling pathway was found in PTC.The expression of Shh/Gli1 was related to the tumor size,clinical stage and lymph node metastasis,indicating that the aberrant activation of Shh signaling pathway plays some roles in PTC.Shh/Gli1 may be indicators for prognosis and ideal targets for therapy against PTC.
