CAJ | 학술논문

目的通过先天性肾盂输尿管连接部梗阻(UPJO)突触素和神经丝蛋白的表达,对UPJO神经肌肉连接进行研究,探讨UPJ0发病机制.方法应用免疫组化技术对20例先天性.肾盂输尿管连接部梗阻患儿和10例肾母细胞瘤患儿的标本在神经肌肉连接处行突触素和神经丝蛋白标记,光镜下观察其免疫反应表达.结果对照组突触素和神经丝蛋白的免疫反应阳性表达,实验组突触素和神经丝蛋白免疫反应呈阴性表达.结论 UPJO狭窄部位突触素和神经丝蛋白的异常表达与UPJO的发生有关,病变段输尿管平滑肌处于失神经支配状态,Cannon定律是UPJO病理生理合理的解释.
목적 통과선천성신우수뇨관련접부경조(UPJO)돌촉소화신경사단백적표체,대UPJO신경기육련접진행연구,탐토UPJ0발병궤제.방법 응용면역조화기술대20례선천성.신우수뇨관련접부경조환인화10례신모세포류환인적표본재신경기육련접처행돌촉소화신경사단백표기,광경하관찰기면역반응표체.결과 대조조돌촉소화신경사단백적면역반응양성표체,실험조돌촉소화신경사단백면역반응정음성표체.결론 UPJO협착부위돌촉소화신경사단백적이상표체여UPJO적발생유관,병변단수뇨관평활기처우실신경지배상태,Cannon정률시UPJO병리생리합리적해석.
Objective To investigate the expressions of synaptophysin and neurofilament in ureteropelvic junction obstruction (UPJO) and to explore the relationship between the expressions of these proteins and the pathogenesis of UPJO. Methods The distribution of the neuromuscular junction from 10 normal controls and 20 ureteropelvic junction obstruction patients were immunostained for synaptophysin and neurofilament.Antimouse synaptophysin and neurofilament serum were applied to identify the neuromuscular junction.Results In the control group,the immunoreactivities of synaptophysin and neurofilament were widely distributed in the muscle layeL In the ureteropelvic junction obstruction group,immunoreactivities of syrmptophysin and neurofilament were absent or moderately positive.Conclusions The reduction of synaptophysin and neurofilament,poor innervation of the ureteral muscles in ureteropelvic junction obstruction possibly results in muscles spasm and poor peristalsis in UPJO.