CAJ | 학술논문

目的为了获得浙江省乙脑病毒基因组详尽的资料,研究基因Ⅰ型乙脑病毒的分子特征及变异程度,为乙脑病毒分子流行病学及其基因研究提供科学依据.方法设计特异性引物、RT-PCR分段扩增XJ69和XJP613株全基因,PER产物纯化后克隆于T载体并进行序列测定.通过生物学软件进行核苷酸序列和氨基酸序列分析和病毒的系统进化分析.结果新分离乙脑病毒XJ69和NJP613株全基因组全长均为10 964个核苷酸,含有一个开放阅读框架,编码3432个氨基酸.与GenBank中选择的32株乙脑病毒全基因序列比较发现,其核苷酸同源为83.5%～99.2%,氨基酸总体同源性为97.5%～99.7%.通过PrM/C区段、E区段及全基因序列进行系统进化分析均显示该毒株属于基因Ⅰ型乙脑病毒.结论新分离的乙脑病毒XJ69和XJP613株属于基因Ⅰ型,与上海三带喙库蚊分离株SH17M-07关系最为接近.
목적 위료획득절강성을뇌병독기인조상진적자료,연구기인Ⅰ형을뇌병독적분자특정급변이정도,위을뇌병독분자류행병학급기기인연구제공과학의거.방법 설계특이성인물、RT-PCR분단확증XJ69화XJP613주전기인,PER산물순화후극륭우T재체병진행서렬측정.통과생물학연건진행핵감산서렬화안기산서렬분석화병독적계통진화분석.결과 신분리을뇌병독XJ69화NJP613주전기인조전장균위10 964개핵감산,함유일개개방열독광가,편마3432개안기산.여GenBank중선택적32주을뇌병독전기인서렬비교발현,기핵감산동원위83.5%～99.2%,안기산총체동원성위97.5%～99.7%.통과PrM/C구단、E구단급전기인서렬진행계통진화분석균현시해독주속우기인Ⅰ형을뇌병독.결론 신분리적을뇌병독XJ69화XJP613주속우기인Ⅰ형,여상해삼대훼고문분리주SH17M-07관계최위접근.
Objective To study the complete genome sequence of Japanese encephalitis virus(JEV) strain X J69 isolated in ZheJiang province and explore its evolution. Methods Overlapping primers were designed according to the fuU-length genomes from C, enBank. RT-PCR was used to amplify the fragments and RT-PCR products were cloned T vector, sequenced and analyzed. Results The genome of strain XJ69 and XJP613 were 10 964 nucleotides in length with a single open reading frame encoding 3432 amino acids. Comparison of the complete geoome sequences of different JEV isolates showed XJ69 and XJP613 were 83.5%-99.2% and 83.4%-99.4% nucleotide sequence homology among them respectively, which resu]ted in 94.8%-99.7% amino acid sequence homology. Phylogetic analysis through PrM/C ,E and full-length genome showed that the X J69 and XJP613 strain belonged to ganotype Ⅰ. Conclusion The nucleoiede sequence and deduced amino acid sequence of X J69 and XJP613 strain were similar to that of those of genotype Ⅰ of Japanese encephalitis virus. It belonged to genotype Ⅰ and were close to the isolates SH17M-07.