CAJ | 학술논문

目的采用不同方法诱导sD清洁级雌性大鼠多囊卵巢综合征(PCOS)动物模型,造模完成后检测大鼠血清相关激素并观察卵巢局部形态学改变,并探讨其意义.方法分别采用来曲唑、硫酸普拉睾酮钠、硫酸普拉睾酮钠联合HCG诱导大鼠PCO模型,RIA法测定血清LH、FSH、E_2、P、T、PRL、INS水平,HE染色后光镜下观察卵巢局部形态.结果 A组(对照组)和B组(来曲唑组)比较,B组较A组血清FSH浓度升高,血清P浓度降低,差异有统计学意义(P<0.05);B组血清T浓度较A组明显升高,差异有显著统计学意义(P<0.01).D组(硫酸普拉睾酮钠组)与C组(对照组)比较,两组间血清性激素及INS差异无显著性(P>0.05).E组(硫酸普拉睾酮钠联合HCG组)与C组比较,E组血清T浓度、LH/FSH比值较C组升高,差异有统计学意义(P<0.05).E组与D组比较,血清P、T浓度升高,差异有统计学意义(P<0.05).A、C两组卵巢局部形态基本正常,可见发育成熟的卵泡及优势卵泡,B、D、E三组未见优势卵泡,均可见卵巢多囊样改变.结论使用来曲唑或硫酸普拉睾酮钠联合HCG诱导大鼠PCOS模型,无论在影响血清性激素还是卵巢局部形态学改变方面与临床表现很接近,符合动物PCOS造模要求.
목적 채용불동방법유도sD청길급자성대서다낭란소종합정(PCOS)동물모형,조모완성후검측대서혈청상관격소병관찰란소국부형태학개변,병탐토기의의.방법 분별채용래곡서、류산보랍고동납、류산보랍고동납연합HCG유도대서PCO모형,RIA법측정혈청LH、FSH、E_2、P、T、PRL、INS수평,HE염색후광경하관찰란소국부형태.결과 A조(대조조)화B조(래곡서조)비교,B조교A조혈청FSH농도승고,혈청P농도강저,차이유통계학의의(P<0.05);B조혈청T농도교A조명현승고,차이유현저통계학의의(P<0.01).D조(류산보랍고동납조)여C조(대조조)비교,량조간혈청성격소급INS차이무현저성(P>0.05).E조(류산보랍고동납연합HCG조)여C조비교,E조혈청T농도、LH/FSH비치교C조승고,차이유통계학의의(P<0.05).E조여D조비교,혈청P、T농도승고,차이유통계학의의(P<0.05).A、C량조란소국부형태기본정상,가견발육성숙적란포급우세란포,B、D、E삼조미견우세란포,균가견란소다낭양개변.결론 사용래곡서혹류산보랍고동납연합HCG유도대서PCOS모형,무론재영향혈청성격소환시란소국부형태학개변방면여림상표현흔접근,부합동물PCOS조모요구.
Objective To establish rat models of polycystic ovary syndrome(PCOS) induced by different methods,to assess the serum levels of several related hormones,to examine the morphological changes in the ovaries,and to discuss their significance.Methods Letrozol,sodium prasterone sulfate,or sodium prasterone sulfate combined with human chorionic gonadotropin were used to establish rat models of PCOS.Radioimmunoassay was used to measure the serum levels of hteinizing hormone(LH),follicle-stimulating hormone(FSH),estrogen(E_2),progesterone(P),testosterone(T),prolactin(PRL),and insulin(INS).HE staining was used to examine the morphological changes of the ovaries.Results Comparing with the normal group A,the serum FSH was increased and the serum progesterone was reduced in the group B,with a statistically significant difference(P<0.05).The serum testosterone was significantly higher in the group B than in the group A(P<0.01).The levels of serum sex hormones and insulin were not significantly different in the group D and C(P>0.05).In comparison with the group C,the levels of serum testosterone and LH/FSH ratio was significantly increased in the group E.(P<0.05).Comparing with the group D,the serum levels of progesterone and testosterone were significantly increased in the group E(P<0.05).The ovaries in the rats of groups A and C showed almost a normal histyology,with mature follicles and dominant follicles.Polycystic changes were observed only in the ovaries of groups B,D and E.Conclusion At the aspect of affecting the level of sex hormones in serum and changing the ovarian morphology.adopting letrozol tablets or sodium prasterone sulfate combined with HCG to induce rat PCO model is more close to clinic manifestations and meets the criteria of PCO animals.In the rat PCOS models induced with letrozol or with sodium prasterone sulfate combined with HCG,either the serum levels of sex hormones and ovarian histology are quite similar to those of human clinical appearance,and may well meet the modeling requirements for future experimental studies of polycystic ovary syndrome.