CAJ | 학술논문

采用打顶法接种、半选择性培养基再分离发病植株中的痛原菌,以及特异性PCR验证方法,对来自3个国家9个不同地区的46株番茄溃疡病菌进行了致病性测定,以病情指数评价不同菌株的致病力.结果显示,分离自我国河北滦平县、内蒙古包头市等地的24株菌株的病情指数达到75以上,属于强致病力水平;11株菌株的病情指数为50～75,属于中等致病力;而9株菌株的病情指教为50以下,属于弱致病力;检测同时证实,有2株属于无致病力菌株.强致病力、中等致病力、弱致病力和无致病力菌株占供试菌株总数的比例分别为52.2%、23.9%、19.6%和4.3%,表明供试的46株番茄溃疡病菌存在不同程度的致病力差异.
채용타정법접충、반선택성배양기재분리발병식주중적통원균,이급특이성PCR험증방법,대래자3개국가9개불동지구적46주번가궤양병균진행료치병성측정,이병정지수평개불동균주적치병력.결과현시,분리자아국하북란평현、내몽고포두시등지적24주균주적병정지수체도75이상,속우강치병력수평;11주균주적병정지수위50～75,속우중등치병력;이9주균주적병정지교위50이하,속우약치병력;검측동시증실,유2주속우무치병력균주.강치병력、중등치병력、약치병력화무치병력균주점공시균주총수적비례분별위52.2%、23.9%、19.6%화4.3%,표명공시적46주번가궤양병균존재불동정도적치병력차이.
The pathogenicity of 46 strains of Clavibacter michiganensis subsp.michiganensis(Cmm),isolated from 9 regions of 3 countries.were tested on tomato seedlings by means of top pruning with inoculation.The bacteria were re-isolated from the infected tomato seedlings by semi-selective medium mSCM and double checked by specific PCR.The results,based on the disease index to assay the pathogenicity difference of tested Cmm strains,showed that 24 of the 46 strains,isolated from Luanping County,Hebei Province and Baotou City,Inner Mongolia,were highly virulent,11 strains moderately virulent,9 strains weakly virulent and the other 2 strains were nonpathogenic.The percentage of each of these four strains were 52.2%,23.9%,19.6%and 4.3%,respectively.The data indicated that forty-six strains of Cmm exhibited significant pathogenicity differences.