临床医学
臨床醫學
림상의학
CLINICAL MEDICINE
2010年
1期
92-94
,共3页
李鹏%谢景华%吴晓钟%高雄辉
李鵬%謝景華%吳曉鐘%高雄輝
리붕%사경화%오효종%고웅휘
腺相关病毒%脑源性神经营养因子%耳聋%顺铂%基因治疗
腺相關病毒%腦源性神經營養因子%耳聾%順鉑%基因治療
선상관병독%뇌원성신경영양인자%이롱%순박%기인치료
Adeno-associated virus%Brain derived neurotrophic factor%Hearing loss%Cisplatin%Gene therapy
目的 探讨腺相关病毒(adeno-associated virus,AAV)作为载体,介导BDNF基因,转染耳蜗细胞后对顺铂致豚鼠耳蜗损害的保护作用.方法 构建rAAV-BDNF,将人工外淋巴液、rAAV-BDNF微注射到顺铂耳聋模型鼠耳蜗内,经脑干诱发电位检测听力阈值及I波潜伏期,ELISA法检测耳蜗外淋巴液BDNF蛋白表达,耳蜗基底膜铺片检测外毛细胞的凋亡.结果实验组和对照组ABR听阈及I波潜伏期、外毛细胞缺失率、BDNF的表达比较差异有统计学意义.结论 AAV能介导BDNF基因转染耳蜗分泌BDNF,拮抗顺铂豚鼠耳蜗毒性,为感音神经性聋的基因治疗提供依据.
目的 探討腺相關病毒(adeno-associated virus,AAV)作為載體,介導BDNF基因,轉染耳蝸細胞後對順鉑緻豚鼠耳蝸損害的保護作用.方法 構建rAAV-BDNF,將人工外淋巴液、rAAV-BDNF微註射到順鉑耳聾模型鼠耳蝸內,經腦榦誘髮電位檢測聽力閾值及I波潛伏期,ELISA法檢測耳蝸外淋巴液BDNF蛋白錶達,耳蝸基底膜鋪片檢測外毛細胞的凋亡.結果實驗組和對照組ABR聽閾及I波潛伏期、外毛細胞缺失率、BDNF的錶達比較差異有統計學意義.結論 AAV能介導BDNF基因轉染耳蝸分泌BDNF,拮抗順鉑豚鼠耳蝸毒性,為感音神經性聾的基因治療提供依據.
목적 탐토선상관병독(adeno-associated virus,AAV)작위재체,개도BDNF기인,전염이와세포후대순박치돈서이와손해적보호작용.방법 구건rAAV-BDNF,장인공외림파액、rAAV-BDNF미주사도순박이롱모형서이와내,경뇌간유발전위검측은력역치급I파잠복기,ELISA법검측이와외림파액BDNF단백표체,이와기저막포편검측외모세포적조망.결과실험조화대조조ABR은역급I파잠복기、외모세포결실솔、BDNF적표체비교차이유통계학의의.결론 AAV능개도BDNF기인전염이와분비BDNF,길항순박돈서이와독성,위감음신경성롱적기인치료제공의거.
Objective To observe the otoprotective efficacy of adeno-associated virus (AAV) as vector mediated brain derived neurotrophic factor(BDNF) gene on cisplatin-induced ototoxicity for cochlear gene therapy. Methods rAAV-BDNF was constructed,and artificial perilymph,rAAV-BDNF were microinjected into the cochlea respectively.The cochlear function was analyzed with evoked auditory brainstem responses(ABR).The BDNF expression was detected with ELISA,and outer hair cells(OHC) were checked for evaluation.Results There was a statistical significance of difference between the two groups in auditory ability, OHC loss,and expression of BDNF.Conclusion AAV-mediated BDNF expression can preserve the cochlear function from the cisplatin-induced ototoxicity.
