浙江大学学报（农业与生命科学版）
浙江大學學報（農業與生命科學版）
절강대학학보（농업여생명과학판）
JOURNAL OF ZHEJIANG UNIVERSITY(AGRICULTURE & LIFE SCIENCES)
2010年
2期
230-236
,共7页
金叶飞%施维林%任岗%韩丽
金葉飛%施維林%任崗%韓麗
금협비%시유림%임강%한려
十二烷基苯磺酸钠(LAS)%镉(Cd)%复合污染%鲫鱼(Carassius auratus)%超氧化物歧化酶(SOD)%过氧化氢酶(CAT)
十二烷基苯磺痠鈉(LAS)%鎘(Cd)%複閤汙染%鯽魚(Carassius auratus)%超氧化物歧化酶(SOD)%過氧化氫酶(CAT)
십이완기분광산납(LAS)%력(Cd)%복합오염%즉어(Carassius auratus)%초양화물기화매(SOD)%과양화경매(CAT)
linear alkylbenzene sulphonates(LAS)%cadmium(Cd)%combined pollution%Carassius auratus%superoxide dismutase (SOD)%catalase (CAT)
采用暴露试验法,研究十二烷基苯磺酸钠(LAS)与重金属Cd~(2+)单独及复合污染对鲫鱼鳃、肝脏、肌肉等部位的Cd蓄积及其超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响.结果表明:单一Cd~(2+)处理时,随着Cd~(2+)浓度增加,鲫鱼鳃、肝脏、肌肉3部位的Cd蓄积量均极显著增加(P<0.01),各组织蓄积量顺序为:肝脏>鳃>肌肉;在LAS与Cd~(2+)复合处理条件下,Cd在这3组织中的分布仍符合以上规律,但鲫鱼各组织对Cd的蓄积量发生改变,其中0.4 mg·L~(-1) Cd~(2+)与5.0 mg·L~(-1) LAS复合处理能极显著促进鲫鱼肝脏对Cd的蓄积(P<0.01);2.0 mg·L~(-1) Cd~(2+)与不同浓度的LAS复合处理均能极显著促进鲫鱼肝脏、肌肉对Cd的蓄积(P<0.01);另外,2.0 mg·L~(-1) Cd~(2+)与5.0 mg·L~(-1) LAS复合处理能极显著促进鲫鱼鳃对Cd的蓄积(P<0.01);其余各复合组与相应单一Cd~(2+)处理组间无显著差异;LAS与Cd~(2+)单一及复合处理对鲫鱼鳃、肝脏、肌肉的SOD、CAT活性均有明显影响,其中鲫鱼肝脏SOD活性变化能更灵敏地反映污染物对鲫鱼的毒性程度.
採用暴露試驗法,研究十二烷基苯磺痠鈉(LAS)與重金屬Cd~(2+)單獨及複閤汙染對鯽魚鰓、肝髒、肌肉等部位的Cd蓄積及其超氧化物歧化酶(SOD)、過氧化氫酶(CAT)活性的影響.結果錶明:單一Cd~(2+)處理時,隨著Cd~(2+)濃度增加,鯽魚鰓、肝髒、肌肉3部位的Cd蓄積量均極顯著增加(P<0.01),各組織蓄積量順序為:肝髒>鰓>肌肉;在LAS與Cd~(2+)複閤處理條件下,Cd在這3組織中的分佈仍符閤以上規律,但鯽魚各組織對Cd的蓄積量髮生改變,其中0.4 mg·L~(-1) Cd~(2+)與5.0 mg·L~(-1) LAS複閤處理能極顯著促進鯽魚肝髒對Cd的蓄積(P<0.01);2.0 mg·L~(-1) Cd~(2+)與不同濃度的LAS複閤處理均能極顯著促進鯽魚肝髒、肌肉對Cd的蓄積(P<0.01);另外,2.0 mg·L~(-1) Cd~(2+)與5.0 mg·L~(-1) LAS複閤處理能極顯著促進鯽魚鰓對Cd的蓄積(P<0.01);其餘各複閤組與相應單一Cd~(2+)處理組間無顯著差異;LAS與Cd~(2+)單一及複閤處理對鯽魚鰓、肝髒、肌肉的SOD、CAT活性均有明顯影響,其中鯽魚肝髒SOD活性變化能更靈敏地反映汙染物對鯽魚的毒性程度.
채용폭로시험법,연구십이완기분광산납(LAS)여중금속Cd~(2+)단독급복합오염대즉어새、간장、기육등부위적Cd축적급기초양화물기화매(SOD)、과양화경매(CAT)활성적영향.결과표명:단일Cd~(2+)처리시,수착Cd~(2+)농도증가,즉어새、간장、기육3부위적Cd축적량균겁현저증가(P<0.01),각조직축적량순서위:간장>새>기육;재LAS여Cd~(2+)복합처리조건하,Cd재저3조직중적분포잉부합이상규률,단즉어각조직대Cd적축적량발생개변,기중0.4 mg·L~(-1) Cd~(2+)여5.0 mg·L~(-1) LAS복합처리능겁현저촉진즉어간장대Cd적축적(P<0.01);2.0 mg·L~(-1) Cd~(2+)여불동농도적LAS복합처리균능겁현저촉진즉어간장、기육대Cd적축적(P<0.01);령외,2.0 mg·L~(-1) Cd~(2+)여5.0 mg·L~(-1) LAS복합처리능겁현저촉진즉어새대Cd적축적(P<0.01);기여각복합조여상응단일Cd~(2+)처리조간무현저차이;LAS여Cd~(2+)단일급복합처리대즉어새、간장、기육적SOD、CAT활성균유명현영향,기중즉어간장SOD활성변화능경령민지반영오염물대즉어적독성정도.
To investigate the combined toxic effects of linear alkylbenzene sulphonates (LAS) and Cd~(2+) in Carassius auratus, Cd accumulations and the activities of superoxide dismutase (SOD) and catalase(CAT) were measured in gill, liver and muscles of C. auratus by exposure to single or combinations of LAS and Cd~(2+). The results indicated that single Cd~(2+) exposure induced its accumulation in all these three tissues with the sequence of liver > gill> muscles. Meanwhile, the combined effects of Cd~(2+) and LAS exhibited a similar pattern in Cd accumulation compared with the single Cd~(2+) exposure in the three tissues. The combination of 0.4 mg·L~(-1) Cd~(2+) and 5.0 mg·L~(-1) LAS induced higher Cd concentration in liver; the combination of 2.0 mg·L~(-1) Cd~(2+) and all the levels of LAS induced higher Cd concentration in liver and muscles; and the combination of 2.0 mg·L~(-1) Cd~(2+) and 5.0 mg·L~(-1) LAS increased higher Cd concentration in gill, as compared with the control. Single or combinations of Cd~(2+) and LAS all influenced the activities of SOD and CAT in three tissues of C. auratus. The changes of SOD activity in liver can reflect better the toxic degree of pollutants to C. auratus.
