CAJ | 학술논문

根据西伯利亚蓼叶片cDNA文库中获得的胚胎发育晚期丰富蛋(late embryogenesis abundant proteins,LEA)基因的部分序列,采用cDNA末端快速扩增(RACE)技术成功克隆了胚胎发育晚期丰富蛋白基因(LEA),命名为PsLEA(GenBank登录号:FJ478172).该基因全长686 bp,5'非翻译区为102 bp,3'非翻译区为131 bp,开放读码框为453 bp,编码150个氨基酸.荧光定量PCR分析表明:正常情况下PsLEA基因在西伯利亚蓼的叶、茎和地下茎中皆有表达,其中茎中表达量最高.3%NaHCO3诱导胁迫下,叶、茎、地下茎中PsLEA表达均受到影响且表达模式存在差异,说明PsLEA基因与西伯利亚蓼的耐盐性相关.
근거서백리아료협편cDNA문고중획득적배태발육만기봉부단(late embryogenesis abundant proteins,LEA)기인적부분서렬,채용cDNA말단쾌속확증(RACE)기술성공극륭료배태발육만기봉부단백기인(LEA),명명위PsLEA(GenBank등록호:FJ478172).해기인전장686 bp,5'비번역구위102 bp,3'비번역구위131 bp,개방독마광위453 bp,편마150개안기산.형광정량PCR분석표명:정상정황하PsLEA기인재서백리아료적협、경화지하경중개유표체,기중경중표체량최고.3%NaHCO3유도협박하,협、경、지하경중PsLEA표체균수도영향차표체모식존재차이,설명PsLEA기인여서백리아료적내염성상관.
Based on a partial sequence of LEA (late embryogenesis abundant proteins) gene from cDNA library of Polygonum sibiricum Laxm.leaves,the LEA gene,designated as PsLEA (GenBank accession No.FJ478172.),was cloned by using rapid amplification of cDNA end (RACE) technology,The acquired gene was 686 bp in full length,including 102 bp of 5' untranslated region and 131 bp of 3' untranslated region.The open reading frame (ORF) is 453 bp in length,encoding a deduced amino acid sequence of 150 residues.Quantitative fluorescent PCR (QF-PCR) results show that PsLEA gene was expressed in leaves and stems as well as underground stems of Polygonum sibiricum Laxm.under normal conditions,and the expression level was higher in stems than in leaves and underground stems.Under 3% NaHCO3 stress,the expression level is greatly affected and the expression patterns varied in different tissues,implying that PsLEA gene plays a role in the salt resistance of Polygonum sibiricum Laxm..