CAJ | 학술논문

[目的]分析杆状病毒泛素蛋白的序列特征和分子进化.[方法] 运用Clustal W软件进行序列多重联配分析,采用临近法(NJ)和最大简约法(MP)构建系统进化树.[结果] 昆虫杆状病毒泛素与真核生物泛素的一致性为73%～86%,差异体现在氨基酸15～32和53～57的序列上,其他序列高度保守.在真核生物泛素蛋白序列中,许多功能性的氨基酸残基在昆虫杆状病毒泛素蛋白序列中也存在.系统进化分析将昆虫杆状病毒和真核生物泛素蛋白大致分为3个亚家族,即GV族、I族和II族.杆状病毒泛素基因采取负选择的进化方式,在长期的进化过程中,其氨基酸序列倾向于逐渐被固定下来,达功能和结构上的稳定.[结论]分析杆状病毒泛素的序列特征和分子进化,为进一步研究其生物学功能提供参考.
[목적]분석간상병독범소단백적서렬특정화분자진화.[방법] 운용Clustal W연건진행서렬다중련배분석,채용림근법(NJ)화최대간약법(MP)구건계통진화수.[결과] 곤충간상병독범소여진핵생물범소적일치성위73%～86%,차이체현재안기산15～32화53～57적서렬상,기타서렬고도보수.재진핵생물범소단백서렬중,허다공능성적안기산잔기재곤충간상병독범소단백서렬중야존재.계통진화분석장곤충간상병독화진핵생물범소단백대치분위3개아가족,즉GV족、I족화II족.간상병독범소기인채취부선택적진화방식,재장기적진화과정중,기안기산서렬경향우축점피고정하래,체공능화결구상적은정.[결론]분석간상병독범소적서렬특정화분자진화,위진일보연구기생물학공능제공삼고.
[Objective] The aim of this study was to analyze the sequence characteristics and molecular evolution of ubiquitins encoded by baculoviruses. [Methods] Clustal W software was used for multiple sequence alignment analysis, and neighbor-joining method (NJ) and maximum parsimony method (MP) were used for the construction of phylogenetic tree. [Results] The baculoviral ubiquitins showed 73%-86% sequence identity to eukaryotic ubiquitin. Two heterogeneous regions of baculoviral ubiquitins were observed: one was the residues from 15-32, the other was located from residues 53 to 60. The else parts were conserved, where many functional amino acids were also observed. Phylogenetic analysis indicated that baculoviral ubiquitins could be divided into three sub-families, including sub-family GV, sub-family I and sub-family II. The molecular evolution of baculoviral ubiquitins might be under negative selection to maintain their functional and structural stability. [Conclusion] The analysis had provided reference for the researches on functional characterization of baculoviral ubiquitins.