中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2010年
6期
562-566
,共5页
张正丰%陈超%王建%周跃%廖维宏
張正豐%陳超%王建%週躍%廖維宏
장정봉%진초%왕건%주약%료유굉
脊髓损伤%基因%治疗%腺病毒科%再生
脊髓損傷%基因%治療%腺病毒科%再生
척수손상%기인%치료%선병독과%재생
Spinal cord injury%Gene%Therapy%Adenoviridae%Regeneration
目的 观察腺病毒介导心肌营养素-1基因(Adv-CT1)转移对大鼠脊髓损伤(SCI)后红核脊髓束(RST)轴突再生和前肢功能恢复的影响.方法 成年Wistar雄性大鼠24只,制备C3脊髓外侧索横切(C3Hx)模型,根据损伤区植入的不同成分分为3组(n=8):损伤对照组(明胶海绵+病毒缓冲液)、Adv-eGFP组(明胶海绵+Adv-eGFP)、Adv-CT1组(明胶海绵+Adv-CT1).荧光金(FG)于C5注射,4周后脑切片荧光显微镜下红核神经元计数观察RST再生;生物素化葡聚糖(BDA)于红核注射,脊髓切片观察RST再生;前肢不对称实验观察行为学的改变.结果 大鼠C3Hx损伤下区注射FG逆行示踪,损伤对照组、Adv-eGFP组和Adv-CT1组标记的红核神经元分别为(12.1±4.3)、(15.2±2.6)和(33.0±5.2)个,Adv-CT1组分别与损伤对照组和Adv-eGFP组比较,差异均有统计学意义(P＜0.05).RST的BDA顺行示踪,损伤对照组、Adv-eGFP组和Adv-CT1组损伤下区BDA标记的神经元分别为(6.0±1.3)、(6.0±1.0)和(17.1±2.0)个,Adv-CT1组分别与损伤对照组和Adv-eGFP组比较,差异均有统计学意义(P＜0.05).大鼠C3Hx4周后AdV-CT1组使用伤肢时间(9.3%±3.3%)明显比损伤对照组(3.6%±1.4%)和Adv-eGFP组(4.1%±2.6%)时间长,差异均有统计学意义(P＜0.05).结论 Adv-CT1转移促进RST再生和SCI后前肢功能部分恢复.
目的 觀察腺病毒介導心肌營養素-1基因(Adv-CT1)轉移對大鼠脊髓損傷(SCI)後紅覈脊髓束(RST)軸突再生和前肢功能恢複的影響.方法 成年Wistar雄性大鼠24隻,製備C3脊髓外側索橫切(C3Hx)模型,根據損傷區植入的不同成分分為3組(n=8):損傷對照組(明膠海綿+病毒緩遲液)、Adv-eGFP組(明膠海綿+Adv-eGFP)、Adv-CT1組(明膠海綿+Adv-CT1).熒光金(FG)于C5註射,4週後腦切片熒光顯微鏡下紅覈神經元計數觀察RST再生;生物素化葡聚糖(BDA)于紅覈註射,脊髓切片觀察RST再生;前肢不對稱實驗觀察行為學的改變.結果 大鼠C3Hx損傷下區註射FG逆行示蹤,損傷對照組、Adv-eGFP組和Adv-CT1組標記的紅覈神經元分彆為(12.1±4.3)、(15.2±2.6)和(33.0±5.2)箇,Adv-CT1組分彆與損傷對照組和Adv-eGFP組比較,差異均有統計學意義(P＜0.05).RST的BDA順行示蹤,損傷對照組、Adv-eGFP組和Adv-CT1組損傷下區BDA標記的神經元分彆為(6.0±1.3)、(6.0±1.0)和(17.1±2.0)箇,Adv-CT1組分彆與損傷對照組和Adv-eGFP組比較,差異均有統計學意義(P＜0.05).大鼠C3Hx4週後AdV-CT1組使用傷肢時間(9.3%±3.3%)明顯比損傷對照組(3.6%±1.4%)和Adv-eGFP組(4.1%±2.6%)時間長,差異均有統計學意義(P＜0.05).結論 Adv-CT1轉移促進RST再生和SCI後前肢功能部分恢複.
목적 관찰선병독개도심기영양소-1기인(Adv-CT1)전이대대서척수손상(SCI)후홍핵척수속(RST)축돌재생화전지공능회복적영향.방법 성년Wistar웅성대서24지,제비C3척수외측색횡절(C3Hx)모형,근거손상구식입적불동성분분위3조(n=8):손상대조조(명효해면+병독완충액)、Adv-eGFP조(명효해면+Adv-eGFP)、Adv-CT1조(명효해면+Adv-CT1).형광금(FG)우C5주사,4주후뇌절편형광현미경하홍핵신경원계수관찰RST재생;생물소화포취당(BDA)우홍핵주사,척수절편관찰RST재생;전지불대칭실험관찰행위학적개변.결과 대서C3Hx손상하구주사FG역행시종,손상대조조、Adv-eGFP조화Adv-CT1조표기적홍핵신경원분별위(12.1±4.3)、(15.2±2.6)화(33.0±5.2)개,Adv-CT1조분별여손상대조조화Adv-eGFP조비교,차이균유통계학의의(P＜0.05).RST적BDA순행시종,손상대조조、Adv-eGFP조화Adv-CT1조손상하구BDA표기적신경원분별위(6.0±1.3)、(6.0±1.0)화(17.1±2.0)개,Adv-CT1조분별여손상대조조화Adv-eGFP조비교,차이균유통계학의의(P＜0.05).대서C3Hx4주후AdV-CT1조사용상지시간(9.3%±3.3%)명현비손상대조조(3.6%±1.4%)화Adv-eGFP조(4.1%±2.6%)시간장,차이균유통계학의의(P＜0.05).결론 Adv-CT1전이촉진RST재생화SCI후전지공능부분회복.
Objective To study whether adenovirus hu cardiotrophin-(CT-1 ) gene transfer promotes rubrospinal axons regeneration and recovery of forelimb function after spinal cord injury (SCI) in adult rats. Methods Tewenty-four adult Wister rats were divided into control, Adv-eGFP and Adv-CT1 groups. Gel foam saturated with different elements was left into a C3-4 lateral funiculus hemisection cavity that completely interrupted one rubrospinal tract(RST) . RST regeneration was measured with FG retrograde and BDA anterograde tracing techniques 4 weeks after lesion. Functional recovery was examined by a forelimb asymmetry test. Results Retrograde tracing with FG showed that the RST neurons regenerated caudal to the injury site were (12. 1 ±4.3), (15.2±2.6) and (33.0 ±5.2) respectively in control, Adv-eGFP and Adv-CT1 groups. Adv-CT1 group was significantly different from control and Adv-eGFP groups ( P ＜ 0. 05) . Anterograde tracing with BDA revealed that the RST axons terminated in the white and gray matter in Adv-CT1 group were ( 17. 1 ±2.0), significantly more than those in control (6. 0 ± 1.3) and Adv-eGFP (6.0 ± 1. 0) groups ( P ＜0. 05) . The behavioral test showed significantly better partial functional recovery in limb usage in Adv-CT1 group (9.3% ±3.3%) than in control (3.6% ±1.4%) and Adv-eGFP (4. 1% ±2.6%) groups (P ＜0. 05) . Conclusions Adv-CT1 transfer promotes rubrospinal axons regeneration and recovery of forelimb function after SCI in adult rats. Recovery of forelimb function may be partially mediated by survival and regeneration of rubrospinal neurons, other descending pathways, and recovery of segmental neuronal circuitry as well.
