中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2010年
11期
937-939
,共3页
脂肪肝%己酮可可碱%线粒体蛋白质类%基因表达
脂肪肝%己酮可可堿%線粒體蛋白質類%基因錶達
지방간%기동가가감%선립체단백질류%기인표체
Fatty liver%Pentoxifylline proteins%Mitochondrial%Gene expression
目的 探讨己酮可可碱(PTX)对非酒精性脂肪肝性肝病(NAFLD)大鼠线粒体解耦联蛋白(UCP)-2 mRNA表达的影响. 方法 SD大鼠60只,正常喂养1周后随机分为3组,每组20只.其中对照组20只,以普通饲料喂养,实验组和干预组各20只,以高脂饲料喂养,干预组高脂饮食4周后,在饮水中加用已酮可可碱(PTX)(100 mg·Kg-1·d-1),于第24周处死,采用反转录聚合酶链反应(RT-PCR)技术检测肝脏UCP-2mRNA的表达. 结果 对照组大鼠肝脏UCP2mRNA呈微量表达(1.2±0.1),实验组大鼠肝脏UCP2mRNA呈强表达(4.0±0.3),干预组大鼠肝脏UCP2mRNA呈弱表达(3.0±0.2),3组间UCP-2mRNA表达差异有统计学意义(F=160.67,P＜0.01). 结论 己酮可可碱可下调NAFLD大鼠肝细胞UCP-2mRNA的表达.
目的 探討己酮可可堿(PTX)對非酒精性脂肪肝性肝病(NAFLD)大鼠線粒體解耦聯蛋白(UCP)-2 mRNA錶達的影響. 方法 SD大鼠60隻,正常餵養1週後隨機分為3組,每組20隻.其中對照組20隻,以普通飼料餵養,實驗組和榦預組各20隻,以高脂飼料餵養,榦預組高脂飲食4週後,在飲水中加用已酮可可堿(PTX)(100 mg·Kg-1·d-1),于第24週處死,採用反轉錄聚閤酶鏈反應(RT-PCR)技術檢測肝髒UCP-2mRNA的錶達. 結果 對照組大鼠肝髒UCP2mRNA呈微量錶達(1.2±0.1),實驗組大鼠肝髒UCP2mRNA呈彊錶達(4.0±0.3),榦預組大鼠肝髒UCP2mRNA呈弱錶達(3.0±0.2),3組間UCP-2mRNA錶達差異有統計學意義(F=160.67,P＜0.01). 結論 己酮可可堿可下調NAFLD大鼠肝細胞UCP-2mRNA的錶達.
목적 탐토기동가가감(PTX)대비주정성지방간성간병(NAFLD)대서선립체해우련단백(UCP)-2 mRNA표체적영향. 방법 SD대서60지,정상위양1주후수궤분위3조,매조20지.기중대조조20지,이보통사료위양,실험조화간예조각20지,이고지사료위양,간예조고지음식4주후,재음수중가용이동가가감(PTX)(100 mg·Kg-1·d-1),우제24주처사,채용반전록취합매련반응(RT-PCR)기술검측간장UCP-2mRNA적표체. 결과 대조조대서간장UCP2mRNA정미량표체(1.2±0.1),실험조대서간장UCP2mRNA정강표체(4.0±0.3),간예조대서간장UCP2mRNA정약표체(3.0±0.2),3조간UCP-2mRNA표체차이유통계학의의(F=160.67,P＜0.01). 결론 기동가가감가하조NAFLD대서간세포UCP-2mRNA적표체.
Objective To investigate the effects of pentoxifylline (PTX) on the expression of uncoupling protein 2 (UCP-2) of hepatic mitochondria in rats with nonalcoholic fatty liver disease (NAFLD). Methods Sixty SD rats were randomly divided into 3 groups (each n = 20): normal control group, experiment group and treatment group. The rats in normal control group were given a normal feed. The rats in experiment and treatment groups were given fat-rich feed. Furthermore, the rats in treatment group were given PTX after 4 weeks in fat-rich diet feeding. The expression of UCP-2 in the liver was detected by immunohistochemistry and semi-quantitative RT-PCR. Results The hepatic expression of UCP2 mRNA was higher in experiment group (4.0±0.3) than in normal control group (1.2±0.1). The hepatic expression of UCP2 mRNA was higher in treatment group (3.0±0.2) than in normal control group, but the hepatic expression of UCP2 mRNA was lower in treatment group than in experiment group (F = 160. 67, P＜ 0. 01). Conclusions The UCP-2 mRNA is expressed in livers of NAFLD, pentoxifylline plays an important role in the reduction of expression of UCP-2 mRNA in lives of NAFLD.
