中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2009年
4期
207-210
,共4页
张孝斌%饶婷%吴天鹏%程帆%葛名欢
張孝斌%饒婷%吳天鵬%程帆%葛名歡
장효빈%요정%오천붕%정범%갈명환
吡咯烷类%肾%再灌注损伤%NF-κB
吡咯烷類%腎%再灌註損傷%NF-κB
필각완류%신%재관주손상%NF-κB
pyrrolidines%kidney%Reperfusion injury%NF-κB
目的 探讨吡咯烷二巯基氨甲酸(PDTC)对大鼠肾缺血再灌注的保护作用及可能的机制.方法 选择成年、健康及雄性的Wistar大鼠56只,随机分为缺血再灌注损伤(IRI)组,PDTC组及对照组.IRI组:24只,建立大鼠肾缺血再灌注模型;PDTC组:24只,缺血再灌注前15 min经鼠尾静脉注射PDTC 150 mg/kg,其余步骤同IRI组;对照组:8只,不给予缺血再灌注处理.IRI组和PDTC组分别于再灌注后2、6和24 h检测大鼠血清肌酐(Cr)和尿素氮(BUN)水平;检测肾组织中自细胞介素8(IL-8)和肿瘤坏死因子α(TNF-α)的含量;逆转录聚合酶链反应(RT-PCR)检测肾组织中核因子-κB(NF-κB)和诱导型一氧化氮合酶(iNOS)mRNA表达水平;苏木素-伊红(HE)染色观察大鼠肾组织的病理变化.取对照组的各项数据作为正常对照.结果 IRI组大鼠再灌注后各时间点的血Cr、BUN、IL-8及TNF-α含量、NF-κB和iNOS mRNA表达水平均高于对照组和PDTC组(P<0.05).再灌注后6 h时,PDTC组大鼠肾组织中IL-8和TNF-α含量与对照组比较,差异无统计学意义(P>0.05).再灌注后24 h时,PDTC组大鼠各项生化指标与对照组相比,差异均无统计学意义(P>0.05).PDTC组大鼠肾损伤的病理变化较IRI大鼠明显减轻.结论 PDTC通过抑制NF-κB,有效减少IL-8,TNFα和iNOS的产生,对肾缺血再灌注有良好的保护作用.
目的 探討吡咯烷二巰基氨甲痠(PDTC)對大鼠腎缺血再灌註的保護作用及可能的機製.方法 選擇成年、健康及雄性的Wistar大鼠56隻,隨機分為缺血再灌註損傷(IRI)組,PDTC組及對照組.IRI組:24隻,建立大鼠腎缺血再灌註模型;PDTC組:24隻,缺血再灌註前15 min經鼠尾靜脈註射PDTC 150 mg/kg,其餘步驟同IRI組;對照組:8隻,不給予缺血再灌註處理.IRI組和PDTC組分彆于再灌註後2、6和24 h檢測大鼠血清肌酐(Cr)和尿素氮(BUN)水平;檢測腎組織中自細胞介素8(IL-8)和腫瘤壞死因子α(TNF-α)的含量;逆轉錄聚閤酶鏈反應(RT-PCR)檢測腎組織中覈因子-κB(NF-κB)和誘導型一氧化氮閤酶(iNOS)mRNA錶達水平;囌木素-伊紅(HE)染色觀察大鼠腎組織的病理變化.取對照組的各項數據作為正常對照.結果 IRI組大鼠再灌註後各時間點的血Cr、BUN、IL-8及TNF-α含量、NF-κB和iNOS mRNA錶達水平均高于對照組和PDTC組(P<0.05).再灌註後6 h時,PDTC組大鼠腎組織中IL-8和TNF-α含量與對照組比較,差異無統計學意義(P>0.05).再灌註後24 h時,PDTC組大鼠各項生化指標與對照組相比,差異均無統計學意義(P>0.05).PDTC組大鼠腎損傷的病理變化較IRI大鼠明顯減輕.結論 PDTC通過抑製NF-κB,有效減少IL-8,TNFα和iNOS的產生,對腎缺血再灌註有良好的保護作用.
목적 탐토필각완이구기안갑산(PDTC)대대서신결혈재관주적보호작용급가능적궤제.방법 선택성년、건강급웅성적Wistar대서56지,수궤분위결혈재관주손상(IRI)조,PDTC조급대조조.IRI조:24지,건립대서신결혈재관주모형;PDTC조:24지,결혈재관주전15 min경서미정맥주사PDTC 150 mg/kg,기여보취동IRI조;대조조:8지,불급여결혈재관주처리.IRI조화PDTC조분별우재관주후2、6화24 h검측대서혈청기항(Cr)화뇨소담(BUN)수평;검측신조직중자세포개소8(IL-8)화종류배사인자α(TNF-α)적함량;역전록취합매련반응(RT-PCR)검측신조직중핵인자-κB(NF-κB)화유도형일양화담합매(iNOS)mRNA표체수평;소목소-이홍(HE)염색관찰대서신조직적병리변화.취대조조적각항수거작위정상대조.결과 IRI조대서재관주후각시간점적혈Cr、BUN、IL-8급TNF-α함량、NF-κB화iNOS mRNA표체수평균고우대조조화PDTC조(P<0.05).재관주후6 h시,PDTC조대서신조직중IL-8화TNF-α함량여대조조비교,차이무통계학의의(P>0.05).재관주후24 h시,PDTC조대서각항생화지표여대조조상비,차이균무통계학의의(P>0.05).PDTC조대서신손상적병리변화교IRI대서명현감경.결론 PDTC통과억제NF-κB,유효감소IL-8,TNFα화iNOS적산생,대신결혈재관주유량호적보호작용.
Objective To investigate the protective mechanism of pyrrolidine dithiocarbamate (PDTC) on the kidney in the isehemia-reperfusion injury model (IRI).Methods Fifty-six health adult Wistar rats were randomly divided into three groups: IRI group (n = 24), PDTC group (n = 24, 150 mg/kg PDTC was administered before IRI), and control group (n = 8).Rats in IRI and PDTC groups were sacrificed at the 2nd, 6th and 24th h after IRI.Serum levels of urea nitrogen (BUN) and creatinine (Cr) were determined.Levels of IL-8, TNF-α, NF-κB and iNOS mRNA was detected in renal tissues.Histological changes were observed.Results At the 2nd, 6th and 24th h after IRI, levels of BUN, Cr, IL-8, TNF-α, NF-κB and iNOS mRNA in IRI group were increased significantly as compared with control and PDTC groups (P<0.05).There was no significant difference in the contents of IL-8 and TNF-α between control and PDTC groups at the 6th h after IRI (P>0.05).No significant differences were found in all parameters between PDTC and control groups at the 24th h after IRI (P>0.05).In comparison to Irl group, renal pathological changes in PDTC group were alleviated significantly.Conclusion These findings indicate that PDTC pretreatment obviously attenuates IRI by down-regulating IL-8, TNF-α and iNOS through inhibiting NF-κB.
