CAJ | 학술논문

目的研究胰腺癌患者外周血CD4-CD8-T细胞比例与IL-4、IFN-γ含量变化及其相互关系.方法分别应用流式细胞仪及ELISA法检测25例胰腺癌患者和45名健康人外周血中CD4-CD8-T细胞比例和IL-4、IFN-γ的含量.结果 25例胰腺癌患者CD4-CD8-T细胞占CD3+T细胞的比例为(4.2±1.7)%,45名健康人CD4-CD8-T细胞占CD3+T细胞的比例为(6.3±2.6)%,两组之间差异有统计学意义(P＜0.01).胰腺癌患者外周血中IL-4含量为(86.3±23.3)fg/L,健康对照组外周血中IL-4含量为(56.2±9.2)fg/L,两组之间差异有统计学意义(P＜0.01).胰腺癌患者外周血中IFN-γ含量为(16.4±4.8)fg/L,健康对照组外周血中IFN-γ含量为(27.4±3.8)fg/L,两组之间差异有统计学意义(P＜0.01).胰腺癌患者手术后CD4-CD8-T细胞比例显著高于术前(P＜0.01).胰腺癌患者外周血CD4-CD8-T细胞比例与IL-4含量呈负相关,与IFN-γ含量呈正相关;胰腺癌患者CD4-CD8-T细胞比例、IL-4含量与肿瘤临床分期有关(P＜0.05),与肿瘤分化程度均无关(P＞0.05),IFN-γ含量与肿瘤临床分期和肿瘤分化程度均无关(P＞0.05).结论 CD4-CD8-T细胞在胰腺癌患者外周血中比例降低,CD4-CD8-T细胞可能通过影响IFN-γ的分泌参与胰腺癌的发生和发展.
목적 연구이선암환자외주혈CD4-CD8-T세포비례여IL-4、IFN-γ함량변화급기상호관계.방법 분별응용류식세포의급ELISA법검측25례이선암환자화45명건강인외주혈중CD4-CD8-T세포비례화IL-4、IFN-γ적함량.결과 25례이선암환자CD4-CD8-T세포점CD3+T세포적비례위(4.2±1.7)%,45명건강인CD4-CD8-T세포점CD3+T세포적비례위(6.3±2.6)%,량조지간차이유통계학의의(P＜0.01).이선암환자외주혈중IL-4함량위(86.3±23.3)fg/L,건강대조조외주혈중IL-4함량위(56.2±9.2)fg/L,량조지간차이유통계학의의(P＜0.01).이선암환자외주혈중IFN-γ함량위(16.4±4.8)fg/L,건강대조조외주혈중IFN-γ함량위(27.4±3.8)fg/L,량조지간차이유통계학의의(P＜0.01).이선암환자수술후CD4-CD8-T세포비례현저고우술전(P＜0.01).이선암환자외주혈CD4-CD8-T세포비례여IL-4함량정부상관,여IFN-γ함량정정상관;이선암환자CD4-CD8-T세포비례、IL-4함량여종류림상분기유관(P＜0.05),여종류분화정도균무관(P＞0.05),IFN-γ함량여종류림상분기화종류분화정도균무관(P＞0.05).결론 CD4-CD8-T세포재이선암환자외주혈중비례강저,CD4-CD8-T세포가능통과영향IFN-γ적분비삼여이선암적발생화발전.
Objective To investigate the relationship between CD4-CD8-T cells ratio and IL-4, IFN-γ levels in the peripheral blood of patients with pancreatic carcinoma. Methods Floweytometer was used to analyze the CD4-CD8-T cells ratio in the peripheral blood of patients with pancreatic carcinoma and the IL-4 ,IFN-γ levels were detected by ELISA. Results The ratio of CD4-CD8-T cell in CD3+ T cell from 25 pancreatic carcinoma patients was(4.2 ± 1.7) %, the ratio of CD4-CD8-T cell in CD3+T cell from 45 healthy person was(6.3 ± 2.6) %, there was significant deviation between the two groups(P ＜ 0. 01). The IL-4 level of 25 pancreatic carcinoma patients was (86.3 ± 23.3) fg/L,the IL-4 level of 45 healthy person was (56.2 ± 9.2) fg/L,there was significant deviation between the two groups(P ＜0.01). The IFN-γ level of 25 pancreatic carcinoma patients was (16.4±4.8) fg/L before operation, the IFN-γ level of 45 healthy person was (27.4±3.8) fg/L,there was significant deviation between the two groups(P ＜0.01). The ratio of CD4-CD8-T cell in pancreatic carcinoma patient after operation was higher than before operation. It could be found negative correlation between CD4-CD8-T cells ratio and IL-4 level in pancreatic carcinoma patient,it could also be found positive correlation between CD4-CD8-T cells ratio and IFN-γ level in pancreatic carcinoma patient. In pancreatic carcinoma patient,the CD4-CD8-T cells ratio and IL-4 level was significant associated with clinical stage (P ＜ 0.05), but no relationship with hisological differentiation (P＞0.05), it could be found no relationship between IFN-γ level and clinical stage, hisoiogieal differentiation (P ＞ 0.05). Conclusion The CD4±CD8±T cells ratio in the peripheral blood of patients is decreased,it may be participate in the carcinogenesis and development of pancreatic carcinoma by influence the IFN-γ levels.