中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2009年
8期
736-741
,共6页
张卫鹏%史学锋%邢咏新%赵堪兴
張衛鵬%史學鋒%邢詠新%趙堪興
장위붕%사학봉%형영신%조감흥
视皮层%神经元%兴奋性突触后电位%受体%AMPA
視皮層%神經元%興奮性突觸後電位%受體%AMPA
시피층%신경원%흥강성돌촉후전위%수체%AMPA
Visual cortex%Neurons%Excitatory postsynaptic potentials%Receptors%AMPA
目的 研究发育过程中大鼠视皮层第2、3层锥体神经元的自发兴奋性突触后电流(sEPSC)变化,探讨生后早期自发性突触活动情况,以及视觉经验在视皮层发育过程中对神经元突触的修饰作用.方法 实验研究.应用红外微分干涉相差显微镜(IR-DIC)结合电耦合式摄像机(CCDCamera)可视法膜片钳全细胞记录生后2~7 d、8~14 d、15~21 d、22~28 d各组sEPSC变化,同时于电极内液中加入0.3%荧光黄对所记录细胞进行染色观察形态学改变.计量资料以均数±标准误表示,经方差齐性检验后,多组样本比较采用单因素方差分析,并进行样本均数间的多重比较.结果 4个组视皮层神经元sEPSC幅值分别为(14.13±0.73)、(15.01±0.62)、(19.87±0.75)、(22.09±1.14)pA,随发育逐渐升高(F=20.69,P<0.01),但2~7 d组与8~14 d组差异无统计学意义(P>0.05),而睁眼前8~14 d组较睁眼后15~21 d组幅值比较差异有统计学意义(P<0.01).4个组视皮层神经元sEPSC频率分别为(1.35±0.05)、(1.33±0.12)、(2.26±0.15)、(2.85±0.12)Hz,随发育逐渐提高(F=87.46,P<0.01),同样睁眼前8~14 d组较睁眼后15~21 d组频率比较差异有统计学意义(P<0.01).视皮层第2、3层神经元胞体及突起以及生物电学特性随发育逐渐成熟.结论 视觉经验对于视皮层第2、3层神经元及突触发育成熟起了关键性作用.发育早期视皮层第2、3层有一定的α-氨基-3-羟基-5甲基-4-异号声(噁)唑丙酸(AMPA)受体功能表达,突触并非完全处于静息状态.
目的 研究髮育過程中大鼠視皮層第2、3層錐體神經元的自髮興奮性突觸後電流(sEPSC)變化,探討生後早期自髮性突觸活動情況,以及視覺經驗在視皮層髮育過程中對神經元突觸的脩飾作用.方法 實驗研究.應用紅外微分榦涉相差顯微鏡(IR-DIC)結閤電耦閤式攝像機(CCDCamera)可視法膜片鉗全細胞記錄生後2~7 d、8~14 d、15~21 d、22~28 d各組sEPSC變化,同時于電極內液中加入0.3%熒光黃對所記錄細胞進行染色觀察形態學改變.計量資料以均數±標準誤錶示,經方差齊性檢驗後,多組樣本比較採用單因素方差分析,併進行樣本均數間的多重比較.結果 4箇組視皮層神經元sEPSC幅值分彆為(14.13±0.73)、(15.01±0.62)、(19.87±0.75)、(22.09±1.14)pA,隨髮育逐漸升高(F=20.69,P<0.01),但2~7 d組與8~14 d組差異無統計學意義(P>0.05),而睜眼前8~14 d組較睜眼後15~21 d組幅值比較差異有統計學意義(P<0.01).4箇組視皮層神經元sEPSC頻率分彆為(1.35±0.05)、(1.33±0.12)、(2.26±0.15)、(2.85±0.12)Hz,隨髮育逐漸提高(F=87.46,P<0.01),同樣睜眼前8~14 d組較睜眼後15~21 d組頻率比較差異有統計學意義(P<0.01).視皮層第2、3層神經元胞體及突起以及生物電學特性隨髮育逐漸成熟.結論 視覺經驗對于視皮層第2、3層神經元及突觸髮育成熟起瞭關鍵性作用.髮育早期視皮層第2、3層有一定的α-氨基-3-羥基-5甲基-4-異號聲(噁)唑丙痠(AMPA)受體功能錶達,突觸併非完全處于靜息狀態.
목적 연구발육과정중대서시피층제2、3층추체신경원적자발흥강성돌촉후전류(sEPSC)변화,탐토생후조기자발성돌촉활동정황,이급시각경험재시피층발육과정중대신경원돌촉적수식작용.방법 실험연구.응용홍외미분간섭상차현미경(IR-DIC)결합전우합식섭상궤(CCDCamera)가시법막편겸전세포기록생후2~7 d、8~14 d、15~21 d、22~28 d각조sEPSC변화,동시우전겁내액중가입0.3%형광황대소기록세포진행염색관찰형태학개변.계량자료이균수±표준오표시,경방차제성검험후,다조양본비교채용단인소방차분석,병진행양본균수간적다중비교.결과 4개조시피층신경원sEPSC폭치분별위(14.13±0.73)、(15.01±0.62)、(19.87±0.75)、(22.09±1.14)pA,수발육축점승고(F=20.69,P<0.01),단2~7 d조여8~14 d조차이무통계학의의(P>0.05),이정안전8~14 d조교정안후15~21 d조폭치비교차이유통계학의의(P<0.01).4개조시피층신경원sEPSC빈솔분별위(1.35±0.05)、(1.33±0.12)、(2.26±0.15)、(2.85±0.12)Hz,수발육축점제고(F=87.46,P<0.01),동양정안전8~14 d조교정안후15~21 d조빈솔비교차이유통계학의의(P<0.01).시피층제2、3층신경원포체급돌기이급생물전학특성수발육축점성숙.결론 시각경험대우시피층제2、3층신경원급돌촉발육성숙기료관건성작용.발육조기시피층제2、3층유일정적α-안기-3-간기-5갑기-4-이호성(오)서병산(AMPA)수체공능표체,돌촉병비완전처우정식상태.
Objective To investigate the modification function of visual experience onto synapses by recording developmental changes of spontaneous excitatory postsynaptic currents(sEPSC)in layer 2 and 3 pyramidal neurons of rat visual cortex.and to observe the spontaneous synapfic activities during the earlier postnataI period.Methods This was an experimental study.By combining infred differential interference contrast(IR-DIC)technique and a CCD-csmera system with visual patch clamp whole-cell recording technique,spontaneous EPSCS of P2~7,P8~14,P15~21 and P22~28 groups were observed and analyzed.The neurons were stained by adding 0.3% Lucifer yellow to the pipette solution simultaneously.Resuits The amplitude of sEPsC was enhanced when aging(F=20.69,P<0.01).The sEPsC frequency increased with age(F=87.46,P<0.01).There was no significant difference between P2~7 groups and P8~14 groups(P>0.05).The eleetrophysiology and dendrite of neuron became mature whilst development.Conclusions During the early posmatal days neurons were relatively immatore.and became mature during development.Visual experience played an important role in this process.In spite of most synapses were taken silent at early postnatal days.and NMDA receptor-mediated funcfion was revealed exclusively,these results suggested that early AMPA receptor-mediated functional activities existed in layer 2 and 3 pyramidal neurons.Synapses were not completely silent,but had early spontaneous activities.