中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2009年
1期
55-58
,共4页
玻璃体视网膜病,增生性%玻璃体脱离%纤溶酶/投药和剂量%透明质酸酶/投药和剂量%动物实验
玻璃體視網膜病,增生性%玻璃體脫離%纖溶酶/投藥和劑量%透明質痠酶/投藥和劑量%動物實驗
파리체시망막병,증생성%파리체탈리%섬용매/투약화제량%투명질산매/투약화제량%동물실험
Vitreoretinopathy,proliferative%Vitreous detachment%Plasmin/administration dosage%Hyaluronoglucosaminidase/administration & dosage%Animal experimentation
目的 观察药物性玻璃体后脱离(PVD)对增生性玻璃体视网膜病变(PVR)的影响.方法 24只有色成年家兔,左眼为实验眼.兔自体富含血小板血浆玻璃体腔注射建立兔眼PVR模型,同时随机将实验兔分为A、B组及对照组,每组各8只眼.建模后3 h A组玻璃体腔内注入1 U纤溶酶(0.05 ml)+20 U透明质酸酶(0.05 m1)共0.1 ml、B组玻璃体腔内注入纤溶酶0.1 ml、对照组玻璃体腔内注入等量的平衡盐溶液.给药后1、7、28 d记录实验眼PVR级别,进行各组PVR等级评分,并行闪光视网膜电图(F-ERG)、眼部B型超声检查及视网膜组织病理学检查.结果 PVR模型成功建立,并在注药后7 d,A组发生完全性PVD 5只眼,不完全性PVD3只眼;B组不完全性PVD 5只眼,未见完全性PVD,另3只及对照组无PVD发生.A、B组在注药后28 d PVR等级评分均低于对照组,差异有统计学意义(D=75.6,98.9;P=0.003,P=0.011);注药后7、28 d,A、B两组F-ERG b波波幅均高于对照组;产生PVD的眼中PVR级别均较无PVD的眼级别低,其中完全性PVD眼中PVR级别仅为0～1级.结论 在兔眼PVR建模后3 h,纤溶酶与透明质酸酶联合玻璃体腔注射诱导的完全性PVD在一定程度上可以阻止兔眼PVR的发生和发展,纤溶酶单独或联合透明质酸酶玻璃体腔注射诱导的不完全性PVD对PVR的发展有减缓作用.
目的 觀察藥物性玻璃體後脫離(PVD)對增生性玻璃體視網膜病變(PVR)的影響.方法 24隻有色成年傢兔,左眼為實驗眼.兔自體富含血小闆血漿玻璃體腔註射建立兔眼PVR模型,同時隨機將實驗兔分為A、B組及對照組,每組各8隻眼.建模後3 h A組玻璃體腔內註入1 U纖溶酶(0.05 ml)+20 U透明質痠酶(0.05 m1)共0.1 ml、B組玻璃體腔內註入纖溶酶0.1 ml、對照組玻璃體腔內註入等量的平衡鹽溶液.給藥後1、7、28 d記錄實驗眼PVR級彆,進行各組PVR等級評分,併行閃光視網膜電圖(F-ERG)、眼部B型超聲檢查及視網膜組織病理學檢查.結果 PVR模型成功建立,併在註藥後7 d,A組髮生完全性PVD 5隻眼,不完全性PVD3隻眼;B組不完全性PVD 5隻眼,未見完全性PVD,另3隻及對照組無PVD髮生.A、B組在註藥後28 d PVR等級評分均低于對照組,差異有統計學意義(D=75.6,98.9;P=0.003,P=0.011);註藥後7、28 d,A、B兩組F-ERG b波波幅均高于對照組;產生PVD的眼中PVR級彆均較無PVD的眼級彆低,其中完全性PVD眼中PVR級彆僅為0～1級.結論 在兔眼PVR建模後3 h,纖溶酶與透明質痠酶聯閤玻璃體腔註射誘導的完全性PVD在一定程度上可以阻止兔眼PVR的髮生和髮展,纖溶酶單獨或聯閤透明質痠酶玻璃體腔註射誘導的不完全性PVD對PVR的髮展有減緩作用.
목적 관찰약물성파리체후탈리(PVD)대증생성파리체시망막병변(PVR)적영향.방법 24지유색성년가토,좌안위실험안.토자체부함혈소판혈장파리체강주사건립토안PVR모형,동시수궤장실험토분위A、B조급대조조,매조각8지안.건모후3 h A조파리체강내주입1 U섬용매(0.05 ml)+20 U투명질산매(0.05 m1)공0.1 ml、B조파리체강내주입섬용매0.1 ml、대조조파리체강내주입등량적평형염용액.급약후1、7、28 d기록실험안PVR급별,진행각조PVR등급평분,병행섬광시망막전도(F-ERG)、안부B형초성검사급시망막조직병이학검사.결과 PVR모형성공건립,병재주약후7 d,A조발생완전성PVD 5지안,불완전성PVD3지안;B조불완전성PVD 5지안,미견완전성PVD,령3지급대조조무PVD발생.A、B조재주약후28 d PVR등급평분균저우대조조,차이유통계학의의(D=75.6,98.9;P=0.003,P=0.011);주약후7、28 d,A、B량조F-ERG b파파폭균고우대조조;산생PVD적안중PVR급별균교무PVD적안급별저,기중완전성PVD안중PVR급별부위0～1급.결론 재토안PVR건모후3 h,섬용매여투명질산매연합파리체강주사유도적완전성PVD재일정정도상가이조지토안PVR적발생화발전,섬용매단독혹연합투명질산매파리체강주사유도적불완전성PVD대PVR적발전유감완작용.
Objective To observe the effect of medicine-induced posterior vitreous detachment (PVD) on proliferative vitreoretinopathy (PVR).Methods.PVR was induced in the left eyes of 24 pigmented rabbits by intravitreal injection with platelet rich plasma.The rabbits were randomly divided into two experimental groups (group A and B) and one control group with 8 eyes in each group.Three hours later,the eyes in group A and B and the control group underwent intravireal injection with 1 U plasmin 0.05 ml+ 20 U hyaluronidase 0.05 ml,plasmin 0.1 ml,and balance salt solution 0.1 ml,respectively.The grade of PVR was recorded 1,7,and 28 days after the intravitreal injection,and the eyes were examined by flash electroretinogram (FERG),B-scan,and retinal histopathological examination.Results The PVR models of rabbit eyes were induced successfully.On the 7th day after injection,complete and partial PVD was found in 5 and 3 eyes respectively in group A;partial PVD in 5 eyes and no complete PVD was observed in group B;there was no PVD in the other 3 eyes in group B and also in the eyes in the control group.On the 28th day after intravitreal injection,PVR grade of group A and B were both obviously lower than that of the control group(D= 75.6,98.9;P = 0.003,P = 0.011) ;On the 7th and 28th day after injection,the b-wave amplitude in group A and B was significantly higher than that in the control group;PVR grade of the PVD eyes was lower than that of non-PVD eyes;PVR grade of the complete PVD eyes was only 0 ～ 1.Conclusions Three hours after the PVR models of rabbit eyes were induced,complete PVD induced by intravitreal injection of plasmin combined with hyaluronidase could prevent the development of PVR of rabbit eyes in some degree;partial PVD induced by plasmin alone or combined with hyaluronidase could relieve the development of PVR.
