中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2008年
8期
868-871
,共4页
叶千红%LI Pei-zhang%曾锐%CHU Yun-zhuo%向瑞屏%ZHOU Tian-hong
葉韆紅%LI Pei-zhang%曾銳%CHU Yun-zhuo%嚮瑞屏%ZHOU Tian-hong
협천홍%LI Pei-zhang%증예%CHU Yun-zhuo%향서병%ZHOU Tian-hong
大肠杆菌%B内酰胺酶类%基因型%澳门
大腸桿菌%B內酰胺酶類%基因型%澳門
대장간균%B내선알매류%기인형%오문
Escherichia coli%β-Lactamase%Genotype%Macao
目的 确定中国澳门地区产超广谱B内酰胺酶(ESBLs)的大肠埃希菌的ESBLs基因型,并与沈阳地区产ESBLs的大肠埃希菌的ESBLs基因型进行比较.方法 分别收集澳门与沈阳地区分离的大肠埃希菌209株、150株.按美国临床和实验室标准协会(CLSI)2006年所定标准确定ESBLs表型,并对ESBLs表型阳性的菌株测试其等电点(pI)值,根据pI值范围设计引物,对其进行PCR扩增,扩增产物测序,测序结果与GenBank比对,确定ESBLs的基因型.结果 (1)澳门与沈阳地区产ESBLs大肠埃希菌阳性检出率分别为30.1%(62/209)、54.O%(81/150).(2)57株(90.5%)澳门产ESBLs的大肠埃希菌基因型为CTX-M,以CTX-M-14为主、占76.2%,并同时发现CTX-M-9、CTX-M-22、CTX-M-27、CTX-M-15、CTX-M-24型,分别占4.8%、3.2%、1.6%、1.6%、3.2%;6株未分型.(3)74株(91.4%)沈阳地区产ESBLs的大肠埃希菌基因型为CTX-M,以CTX-M-14为主、占65.4%,发现CTX-M-3、CTX.M-24、CTX-M-22、CTX-M-15、CTX-M-9、CTX-M-28型,分别占13.6%、4.9%、2.5%、2.5%、1.2%、1.2%;7株未分型.结论 澳门产ESBLs大肠埃希菌基因型为CTX-M型,以CTX-M-14为主,同时存在CTX-M-15、CTX-M-22、CTX-M-24、CTX-M-9、CTX-M-27型,基因型与沈阳地区菌株基本相同,未发现CTX-M-3、CTX-M-28型.沈阳地区菌株未发现CTX-M-27型.
目的 確定中國澳門地區產超廣譜B內酰胺酶(ESBLs)的大腸埃希菌的ESBLs基因型,併與瀋暘地區產ESBLs的大腸埃希菌的ESBLs基因型進行比較.方法 分彆收集澳門與瀋暘地區分離的大腸埃希菌209株、150株.按美國臨床和實驗室標準協會(CLSI)2006年所定標準確定ESBLs錶型,併對ESBLs錶型暘性的菌株測試其等電點(pI)值,根據pI值範圍設計引物,對其進行PCR擴增,擴增產物測序,測序結果與GenBank比對,確定ESBLs的基因型.結果 (1)澳門與瀋暘地區產ESBLs大腸埃希菌暘性檢齣率分彆為30.1%(62/209)、54.O%(81/150).(2)57株(90.5%)澳門產ESBLs的大腸埃希菌基因型為CTX-M,以CTX-M-14為主、佔76.2%,併同時髮現CTX-M-9、CTX-M-22、CTX-M-27、CTX-M-15、CTX-M-24型,分彆佔4.8%、3.2%、1.6%、1.6%、3.2%;6株未分型.(3)74株(91.4%)瀋暘地區產ESBLs的大腸埃希菌基因型為CTX-M,以CTX-M-14為主、佔65.4%,髮現CTX-M-3、CTX.M-24、CTX-M-22、CTX-M-15、CTX-M-9、CTX-M-28型,分彆佔13.6%、4.9%、2.5%、2.5%、1.2%、1.2%;7株未分型.結論 澳門產ESBLs大腸埃希菌基因型為CTX-M型,以CTX-M-14為主,同時存在CTX-M-15、CTX-M-22、CTX-M-24、CTX-M-9、CTX-M-27型,基因型與瀋暘地區菌株基本相同,未髮現CTX-M-3、CTX-M-28型.瀋暘地區菌株未髮現CTX-M-27型.
목적 학정중국오문지구산초엄보B내선알매(ESBLs)적대장애희균적ESBLs기인형,병여침양지구산ESBLs적대장애희균적ESBLs기인형진행비교.방법 분별수집오문여침양지구분리적대장애희균209주、150주.안미국림상화실험실표준협회(CLSI)2006년소정표준학정ESBLs표형,병대ESBLs표형양성적균주측시기등전점(pI)치,근거pI치범위설계인물,대기진행PCR확증,확증산물측서,측서결과여GenBank비대,학정ESBLs적기인형.결과 (1)오문여침양지구산ESBLs대장애희균양성검출솔분별위30.1%(62/209)、54.O%(81/150).(2)57주(90.5%)오문산ESBLs적대장애희균기인형위CTX-M,이CTX-M-14위주、점76.2%,병동시발현CTX-M-9、CTX-M-22、CTX-M-27、CTX-M-15、CTX-M-24형,분별점4.8%、3.2%、1.6%、1.6%、3.2%;6주미분형.(3)74주(91.4%)침양지구산ESBLs적대장애희균기인형위CTX-M,이CTX-M-14위주、점65.4%,발현CTX-M-3、CTX.M-24、CTX-M-22、CTX-M-15、CTX-M-9、CTX-M-28형,분별점13.6%、4.9%、2.5%、2.5%、1.2%、1.2%;7주미분형.결론 오문산ESBLs대장애희균기인형위CTX-M형,이CTX-M-14위주,동시존재CTX-M-15、CTX-M-22、CTX-M-24、CTX-M-9、CTX-M-27형,기인형여침양지구균주기본상동,미발현CTX-M-3、CTX-M-28형.침양지구균주미발현CTX-M-27형.
Objective To identify the genotypos of extended spectrum β-1actamase (ESBLs)-producing of Escherichia coli ( E. coli) clinical strains isolated from the Macao and compare the results with the genotypes of clinical strains collected in the first Clinical College, China Medical University (CMU) in Shengyang. Methods The clinically isolated E. coli strains including 209 strains from Macao and 150 strains from CMU were collected. Based on the standard of CLSI2006, the ESBLs-producing strains was identified and its isoelectric point(pI) value was detected by isoelectric focusing (IEF) method. The pI values were used to design the primers for PCR amplification. The amplified DNA sequences were then compared with the GenBank and the ESBL genotypes were confirmed. Results ( 1 ) The positive rate of ESBLs-producing strains of E. coli was 30. 1% (62/209) from Macao and 54. 0% (81/150)from CMU. (2)The genotype of 56 (90. 5% ) β-lactamase(ESBLs)-producing E. cull strains from Macao was CTX-M56. Most of them were CTX-M-14 (76. 2% ), other genotypes including CTX-M-9 (4. 8% ), CTX-M-22 (3.2%), CTX-M-24(3.2%), CTX-M-27(1. 6% ), and CTX-M-15( 1.6% ) were found. Six strains were unidentified. (3)The genotype of 74(91.5% )β-lactamase(ESBLs) -producing E. coli strains from Shenyang was CTX-M. Most of them were CTX-M-14 (65.4%), other genotypes including CTX-M-3 ( 13.6% ), CTX-M-24 (4. 9% ),CTX-M-22(2.5%), CTX-M-15(2.5%), CTX-M-9(1.2%) and CTX-M-28(1.2%) were found. Seven strains were unidentified. Conclusions CTX-M genotypo was the mostly identified ESBL-preducing E. Coli strains from Macao and the results were similar with that from CMU. Among them, the CTX-M-14 was the major genotype. Other genotypes included CTX-M-9, CTX-M-15, CTX-M-22, CTX-M-27, and CTX-M-24.However, two genotypes of CTX-M-3 and CTX-M-28 were not found in the clinical isolates in Macao and one genotype of CTX-M-27 was not found from the CMU clinical isolates.
