CAJ | 학술논문

采用自组装技术,将5'端标记有巯基的20-merODN(oligo-1)以金-硫键形式牢固结合在7.995MHz的AT-切石英晶体的镀金表面,然后由石英晶体微天平实时检测了与碱基序列互补的10-mer ODN (oligo-2)和 8-mer ODN(oligo-3)的杂交,同时还研究了稀土金属铈离子在温和条件下对DNA的水解切断作用.结果表明:应用QCM方法可能实时检测DNA的固定和杂交,Ce(IV)能随机切断单链DNA;但不能切断杂交形成的双链DNA,因此可利用杂交保护的方法对单链DNA实行定位切断.
채용자조장기술,장5'단표기유구기적20-merODN(oligo-1)이금-류건형식뢰고결합재7.995MHz적AT-절석영정체적도금표면,연후유석영정체미천평실시검측료여감기서렬호보적10-mer ODN (oligo-2)화 8-mer ODN(oligo-3)적잡교,동시환연구료희토금속시리자재온화조건하대DNA적수해절단작용.결과표명:응용QCM방법가능실시검측DNA적고정화잡교,Ce(IV)능수궤절단단련DNA;단불능절단잡교형성적쌍련DNA,인차가이용잡교보호적방법대단련DNA실행정위절단.
The use of quartz crystal microbalance (QCM) for in-situ monitoring the immobilization of single-stranded DNA(oligo-1), marked with mercaptol group at 5' end, on the surface of gold-filled 7.995 MHz AT-cut quartz crystal by Au-S bond with self-assemble technique is reported. And then the hybridization of ssDNA with the complementary 10-mer ODN(oligo-2) and 8-mer ODN(oligo-3) is described. Moreover, the QCM has been employed to analyze the DNA cleavage by cerium(IV) ions under moderate conditions. The results showed that the QCM, which is capable of sensitive measurement, was able to in-situ investigate immobilization, hybridization and cleavage of ssDNA. And the cerium(IV) ions had no cleavage for the double-stranded DNA, but it could hydrolyze single-stranded DNA. So the hydrolytic cleavage of ssDNA at the specific site could be realized by the protection of the hybridization.