干旱地区农业研究
榦旱地區農業研究
간한지구농업연구
AGRICULTURAL RESEARCH IN THE ARID AREAS
2009年
4期
111-115,135
,共6页
张春梅%邹志荣%黄志%张志新
張春梅%鄒誌榮%黃誌%張誌新
장춘매%추지영%황지%장지신
钙调素拮抗剂W7%干旱胁迫%番茄%抗氧化系统
鈣調素拮抗劑W7%榦旱脅迫%番茄%抗氧化繫統
개조소길항제W7%간한협박%번가%항양화계통
calmodulin antagonist W7%PEG stress%tomato%antioxi datant system
以耐旱性不同的2个番茄品种为试材,采用营养液水培法,研究了钙、钙调素拮抗剂W7[N-(6-aminohexyl) -5-chloro-1-naphthalene sulfonami de]预处理对聚乙二醇模拟干旱胁迫下番茄幼苗抗氧化系统的影响.结果表明,钙调素拮抗剂W7浸种处理显著提高了番茄幼苗丙二醛(MDA)、H2O2含量和O2 ()/(*)]产生速率,抑制了超氧化物歧化酶(SOD)、过氧化物酶(POD)活性,加剧了抗坏血酸(A sA)和还原型谷胱甘肽(GSH)的破坏;而Ca 2+处理显著降低了PEG胁迫下番茄幼苗MDA 、H2O2含量和O2 ()/(*)产生速率,提高了SOD、POD活性,减轻了胁迫对AsA和GSH的破坏.相同处理条件下,耐旱性强的品种'毛粉802'较耐旱性弱的品种'皇冠'伤害程度轻,Ca 2+-CaM信号系统对缓解番茄PEG胁迫具有重要作用.
以耐旱性不同的2箇番茄品種為試材,採用營養液水培法,研究瞭鈣、鈣調素拮抗劑W7[N-(6-aminohexyl) -5-chloro-1-naphthalene sulfonami de]預處理對聚乙二醇模擬榦旱脅迫下番茄幼苗抗氧化繫統的影響.結果錶明,鈣調素拮抗劑W7浸種處理顯著提高瞭番茄幼苗丙二醛(MDA)、H2O2含量和O2 ()/(*)]產生速率,抑製瞭超氧化物歧化酶(SOD)、過氧化物酶(POD)活性,加劇瞭抗壞血痠(A sA)和還原型穀胱甘肽(GSH)的破壞;而Ca 2+處理顯著降低瞭PEG脅迫下番茄幼苗MDA 、H2O2含量和O2 ()/(*)產生速率,提高瞭SOD、POD活性,減輕瞭脅迫對AsA和GSH的破壞.相同處理條件下,耐旱性彊的品種'毛粉802'較耐旱性弱的品種'皇冠'傷害程度輕,Ca 2+-CaM信號繫統對緩解番茄PEG脅迫具有重要作用.
이내한성불동적2개번가품충위시재,채용영양액수배법,연구료개、개조소길항제W7[N-(6-aminohexyl) -5-chloro-1-naphthalene sulfonami de]예처리대취을이순모의간한협박하번가유묘항양화계통적영향.결과표명,개조소길항제W7침충처리현저제고료번가유묘병이철(MDA)、H2O2함량화O2 ()/(*)]산생속솔,억제료초양화물기화매(SOD)、과양화물매(POD)활성,가극료항배혈산(A sA)화환원형곡광감태(GSH)적파배;이Ca 2+처리현저강저료PEG협박하번가유묘MDA 、H2O2함량화O2 ()/(*)산생속솔,제고료SOD、POD활성,감경료협박대AsA화GSH적파배.상동처리조건하,내한성강적품충'모분802'교내한성약적품충'황관'상해정도경,Ca 2+-CaM신호계통대완해번가PEG협박구유중요작용.
The effect of Ca 2+ and calmodulin antagonist W7 on antioxidant systems of tomato (Lycopersicon esculentum M.) seedlings under drought stress induced by 10% polyethylene glycol (PEG-6000) was studied i n hydroponics culture. The results showed that pretreated seedlings with calmodul in antagonist W7 under PEG stress had lower SOD and POD activities, and higher p roduction rate of O2 ()/(*), MDA and H2O2, in addition, ag gravated the dam age of AsA and GSH; On the contrary, Ca 2+ treatment enabled the seedli ngs to keep relatively high activities of SOD and POD, lower production rate of O 2-* and MDA content, at the same time, alleviated the accumulation of AsA a nd GSH. These results indicated that Ca 2+-CaM signal transduction might re gulate resistance of tomato seedlings to PEG stress of tomato seedlings by affec ting the activity of some antioxidant enzymes and the content of antioxidant sub stance. The experiment shows that Ca 2+-CaM signal system plays an importan t regulating role in alleviating PEG stress induced by 10% polyethylene glycol ( PEG-6000) in seedlings.