CAJ | 학술논문

[目的]测定陕西产延胡索中延胡索乙素、原阿片碱的含量.[方法]采用HPLC法,色谱柱为Agilent ODSC18色谱柱(150 mm×4.6 mm,5 μm),流动相为0.1%磷酸缓冲液-甲醇(65∶35,三乙胺调pH值至6.0),检测波长为280 nm,流速为1.0 ml/min,柱温为25 ℃,进样量10 μl.[结果]陕西产延胡索中延胡索乙素、原阿片碱的平均含量分别为0.977和0.651 mg/g,2种成分平均加样回收率分别为98.02%(RSD=1.87%)和98.63%(RSD=1.43%).[结论]陕西产延胡索中延胡索乙素、原阿片碱含量与其他文献资料相比较高,符合2005版药典含量要求.
[목적]측정협서산연호색중연호색을소、원아편감적함량.[방법]채용HPLC법,색보주위Agilent ODSC18색보주(150 mm×4.6 mm,5 μm),류동상위0.1%린산완충액-갑순(65∶35,삼을알조pH치지6.0),검측파장위280 nm,류속위1.0 ml/min,주온위25 ℃,진양량10 μl.[결과]협서산연호색중연호색을소、원아편감적평균함량분별위0.977화0.651 mg/g,2충성분평균가양회수솔분별위98.02%(RSD=1.87%)화98.63%(RSD=1.43%).[결론]협서산연호색중연호색을소、원아편감함량여기타문헌자료상비교고,부합2005판약전함량요구.
[Objective]The research aimed to establish a method for determination of d1-Tetrahydropalmatine and protopine in Corydalis yanhusuo.[Method]HPLC separation was carried on a column of ODS (4.6 mm×150 mm, 5 μm), with the mobile phase of 0.1% phosphate buffer-methanol (65∶35) at the flow rate of 1.0 ml/min, and the detection wavelength was set at 280 nm.[Result]The average content of d1-Tetrahydropalmatine and protopine in Corydalis yanhusuo produced from was 0.977 and 0.651 mg/g respectively.Their average recovery were 98.02% (RSD=1.87%) and 98.63% (RSD=1.43%).[Conclusion]Compared with other literatures, the content of two active components in Corydalis yanhusuo W.T.Wang from Shaanxi, the content of d1-Tetrahydropalmatine and protopine were relative higher, which could meet the content requirements of China Pharmacopeia (2005).