CAJ | 학술논문

采用响应面法对獐牙菜苦苷的生物转化条件进行优化,首先应用Plackett-Burman方法筛选出对转化具有显著性影响的因素,再用中心响应面法的Response Optimizer工具对最优转化条件进行了分析和预测,最后对预测结果进行了验证.结果表明,獐牙菜新碱的最优化转化条件(mg/mL)为MgSO_4·7H_2O 5.14、MnSO_4·4H_2O 3.42、葡萄糖 9.95、蛋白胨9.24、pH 5.80.在此条件下獐牙菜新碱的最大转化产率为17.64%;红百金花内酯的最优转化条件(mg/mL)为MgSO_4·7H_2O 4.09、MnSO_4·4H_2O 6.07、葡萄糖 7.37、酵母膏7.37,pH 5.73.在此条件下红百金花内酯的最大转化产率为8.81%.
채용향응면법대장아채고감적생물전화조건진행우화,수선응용Plackett-Burman방법사선출대전화구유현저성영향적인소,재용중심향응면법적Response Optimizer공구대최우전화조건진행료분석화예측,최후대예측결과진행료험증.결과표명,장아채신감적최우화전화조건(mg/mL)위MgSO_4·7H_2O 5.14、MnSO_4·4H_2O 3.42、포도당 9.95、단백동9.24、pH 5.80.재차조건하장아채신감적최대전화산솔위17.64%;홍백금화내지적최우전화조건(mg/mL)위MgSO_4·7H_2O 4.09、MnSO_4·4H_2O 6.07、포도당 7.37、효모고7.37,pH 5.73.재차조건하홍백금화내지적최대전화산솔위8.81%.
Biotransformation conditions of swertiamarin were optimized by response surface methodology. The Plackett-Burman method was firstly used to screen significant factors on the biotransformation. Central response surface method was used to analyze the biotransformation and the response optimizer tool was used to estimate the optimal biotransformation conditions of swertiamarin to alkaloid and erythrocentaurin, respectively. Finally, the optimal conditions were verified by fermentation test. The optimal condition for the alkaloid formation was (in mg/mL): MgSO_4·7H_2O 5.14, MnSO_4·4H_2O 3.42, glucose 9.95, peptone 9.24, and pH 5.80, the maximum recovery was 17.64%. The optimal condition for erythrocentaurin was (in mg/mL): MgSO_4·7H_2O 4.09, MnSO_4·4H_2O 6.07, glucose 7.37, peptone 7.37, and pH 5.73, the maximum recovery was 8.81%.