CAJ | 학술논문

多酚氧化酶是一种氧化还原酶，主要与植物色素的生成及其产品的色变有关，有利于水果或作物营养价值的保持．本论文以绿豆嫩叶多酚氧化酶粗提液为研究对象，采用不同条件下的磁感应强度的稳恒磁场处理，研究其对多酚氧化酶活性的影响．实验过程分别选用不同的磁感应强度以及时间处理，结果表明：在测酶活性过程中，酶与底物反映很快，1min时底物已全部反应，吸光度也不再增加．在反应初，对照组吸光度为0．8822．处理条件为1．6T15min时，磁场处理对PPO活性促进作用最明显，吸光度为1．0023．反应初始阶段完成以后，对照组酶活力为0．668U．处理条件为0．8T10min时，磁场处理对PPO活性促进作用最明显，酶活力为0．781U．
다분양화매시일충양화환원매，주요여식물색소적생성급기산품적색변유관，유리우수과혹작물영양개치적보지．본논문이록두눈협다분양화매조제액위연구대상，채용불동조건하적자감응강도적은항자장처리，연구기대다분양화매활성적영향．실험과정분별선용불동적자감응강도이급시간처리，결과표명：재측매활성과정중，매여저물반영흔쾌，1min시저물이전부반응，흡광도야불재증가．재반응초，대조조흡광도위0．8822．처리조건위1．6T15min시，자장처리대PPO활성촉진작용최명현，흡광도위1．0023．반응초시계단완성이후，대조조매활력위0．668U．처리조건위0．8T10min시，자장처리대PPO활성촉진작용최명현，매활력위0．781U．
The polyphenol oxidase is an oxidoreductase, mainly related to plant pigment formation and its products, the discoloration is conducive to fruit or crops to maintain nutritional value. This paper has true leaf polyphenol oxidase in mung bean crude extracts were studied, using different conditions of magnetic in duction intensity static magnetic field treatment on the polyphenol oxidase activity to study its impacts. The experiment were selected to the different magnetic field strength as well as the time to deal with, results showed that： in the process of measuring enzyme activity, enzyme and substrate to reflect rapidly around the substrate have been close to lmin reaction, the absorbance no longer increased. In response to the be- ginning of the control group absorbance of 0. 8822. Processing conditions 1.6 T15 rain, the magnetic field treatment on the PPO activity in the promotion of the role of the most significant absorbance of 1. 0023. After the initial response phase is completed, the control group for enzyme activity 0.66.8U. Processing condi- tions 0.8 T 10 rain,the magnetic field treatment on the PPO activity of the most significant role in promoting the enzyme activity of 0. 781 U.