CAJ | 학술논문

目的了解甲型流感病毒流行株的血凝素基因变异和抗原变异及两者间的关系,结合流行病学资料分析基因变异和抗原变异的吻合情况.方法从2005-2007年上海市季节性流感样病例分离到的甲型流感病毒株中,选取H1N1、H3N2两个亚型中部分有代表性的病毒株,并同WHO北半球流感疫苗推荐株一起,进行血凝素全基因序列测定后做基因进化树分析.同时用灭活的全病毒抗原免疫金黄地鼠,通过血凝抑制试验测定病毒的血凝效价,对血凝抑制结果进行聚类分析,绘制病毒的抗原变异图.结果 H3N2分离株与WHO北半球疫苗推荐株A/Sydney/5/97、A/Fujian/411/2002处于不同的基因进化分枝上,时间间隔越久,进化距离越远,同样的结果也出现在抗原变异分析中.而H1N1分离株的基因变异情况和抗原变异情况则不一致,在基因变异中,与疫苗推荐株A/New Caledonia/20/1999的距离远近受到分离时间的影响,抗原变异还与病毒是否从散发病例或聚集性病例分离有关.结论流感病毒血凝素的基因变异和抗原变异的结果基本吻合,采用流行株免疫血清的血凝抑制试验能更好地反映病毒的变异和进化情况.
목적 료해갑형류감병독류행주적혈응소기인변이화항원변이급량자간적관계,결합류행병학자료분석기인변이화항원변이적문합정황.방법 종2005-2007년상해시계절성류감양병례분리도적갑형류감병독주중,선취H1N1、H3N2량개아형중부분유대표성적병독주,병동WHO북반구류감역묘추천주일기,진행혈응소전기인서렬측정후주기인진화수분석.동시용멸활적전병독항원면역금황지서,통과혈응억제시험측정병독적혈응효개,대혈응억제결과진행취류분석,회제병독적항원변이도.결과 H3N2분리주여WHO북반구역묘추천주A/Sydney/5/97、A/Fujian/411/2002처우불동적기인진화분지상,시간간격월구,진화거리월원,동양적결과야출현재항원변이분석중.이H1N1분리주적기인변이정황화항원변이정황칙불일치,재기인변이중,여역묘추천주A/New Caledonia/20/1999적거리원근수도분리시간적영향,항원변이환여병독시부종산발병례혹취집성병례분리유관.결론 류감병독혈응소적기인변이화항원변이적결과기본문합,채용류행주면역혈청적혈응억제시험능경호지반영병독적변이화진화정황.
Objective To study the correlation between genetic diversity and antigenic variation of hemagglutinin in influenza A viruses and analyze the coincidence of these two variations with epidemiological data. MethodsA/H1N1 and A/H3N2 influenza viruses isolated from flu-like cases in Shanghai and surrounding areas during 2005-2007 influenza seasons as well as some vaccine strains for northern hemisphere recommended by WHO were used. The correlation between genetic diversity and antigenic variation of hemagglutinin in influenza A viruses was studied. Full-length sequence encoding hemagglutinin was determined and analyzed by phylogenetic tree analysis. Golden hamsters were immunized with inactivated influenza viruses, and their sera were titrated for the virus hemagglutinin by hemagg/utination inhibition assay (HIA). HIA data were clustered and mapped. Results A/H3N2 viruses isolated in Shanghai and WHO recommended vaccine strains, such as A/Sydney/5/97-like and A/Fujian/411/2002-like, were located at different clusters. The distance was associated with time when the virus was isolated. The more the interval time between isolations, the longer was the distance. Similar trend was found in antigenic variation. In A/H1N1 strains, however, the antigenic variation was not in accordance with the genetic diversity. The distances of genetic diversity between Shanghai isolates and the WHO recommended vaccine strain, A/New Caledonla/20/1999-like, were influenced by time. Whereas, the distance of antigenic variation was not only associated with the time of isolation but also with the virus strains isolated from sporadic or clustered cases. Conclusions The genetic diversity of hemagglutinin is similar to antigenic variation of hemagglutinin in influenza A viruses, and the mutation and evolution of hemagglutinin antigen can be better reflected with HIA by using the immune sera of epidemic strains.