CAJ | 학술논문

目的比较五种方法检测外周血嗜酸性粒细胞计数的准确性.方法对100例健康体检正常者分别采用显微镜直接计数、三种不同产地的五分类血细胞自动分析仪、流式细胞仪等五种方法进行外周血嗜酸性粒细胞检测.结果 100例血标本检测结果:显微镜计数法为(0.249±0.1]2)×109/L;BC-5500、XE-2100和AC·T5diff血细胞分析仪所测结果分别为(0.253±0.105)×109/L、(0.252±0.103)×109/L和(0.256±0.104)×109/L;流式细胞仪所测结果为(0.259±0.101)×109/L,五种方法结果差异无统计学意义(F=1.225,P＞0.10).结论五种方法检测外周血嗜酸性粒细胞计数均较准确,五分类血细胞自动分析仪和流式细胞仪操作简便,重复性好,但仪器设备及试剂较昂贵;显微镜直接计数方法操作较烦琐,重复性稍差.
목적 비교오충방법검측외주혈기산성립세포계수적준학성.방법 대100례건강체검정상자분별채용현미경직접계수、삼충불동산지적오분류혈세포자동분석의、류식세포의등오충방법진행외주혈기산성립세포검측.결과 100례혈표본검측결과:현미경계수법위(0.249±0.1]2)×109/L;BC-5500、XE-2100화AC·T5diff혈세포분석의소측결과분별위(0.253±0.105)×109/L、(0.252±0.103)×109/L화(0.256±0.104)×109/L;류식세포의소측결과위(0.259±0.101)×109/L,오충방법결과차이무통계학의의(F=1.225,P＞0.10).결론 오충방법검측외주혈기산성립세포계수균교준학,오분류혈세포자동분석의화류식세포의조작간편,중복성호,단의기설비급시제교앙귀;현미경직접계수방법조작교번쇄,중복성초차.
Objective To comparisom five methods of detection of eosinophil cells,in order to analyze the precision of the five methods.Methods The eosinophil cells of 100 patients were tested by using microscope、BC T5difft and flow cytometry are(0.249 ±0.112) × 109/L,(0.253 ±0.105) × 109/L,(0.252 ±0.103) × 109/L,(0.256 ±0.104) × 109/L and(0.259 ±0.101) × 109/L.There are no statistical differences(F = 1.225,P＞0.10)among the five methods.Conclusion The results of the five methods are all accurate;the automatic analyzer method and the flow cytometry method are simple and repeatable,but the equipment and reagents are more expensive;the microscope direct counting method is more complicated and less repeatable.