中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
6期
808-809
,共2页
闫涛%俞建军%李成海%姜应传%谷宝军%徐月敏
閆濤%俞建軍%李成海%薑應傳%穀寶軍%徐月敏
염도%유건군%리성해%강응전%곡보군%서월민
精索静脉曲张%睾丸酶%增殖细胞核抗原%不育
精索靜脈麯張%睪汍酶%增殖細胞覈抗原%不育
정색정맥곡장%고환매%증식세포핵항원%불육
Varicocele%Testis enzyme%Proliferating cell nuclear antigen%Infertility
目的 观察改进型左侧精索静脉曲张(ELV)对大鼠睾丸酶及增殖细胞核抗原(PCNA)的影响.方法 利用改进的方法建立青春期SD大鼠左精索静脉曲张的模型21只;假手术组SD大鼠15只作对照组.术后3个月,取部分睾丸组织匀浆提取上清液,比色法定量检测乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、葡萄糖-6-磷酸脱氢酶(G6PDH)活性,利用酶联免疫吸附试验(ELISA)测定PCNA的浓度.结果 实验组左睾丸内的LDH、G6PDH活性分别为(8.17±3.47)、(34.00±16.29)U/mg,与对照组同侧(11.98±2.26)、(54.88±20.87)U/mg比较下降,与实验组右睾丸(12.69±3.97)、(78.03±25.28)U/mg比较也下降,差异均有统计学意义(P<0.05);实验组左睾丸内的SDH活性(1.22±0.41)U/mg与对照组同侧(1.74±0.43)U/mg比较下降,差异有统计学意义(P<0.05),与实验组右睾丸(1.62±0.56)U/mg比较下降,差异无统计学意义(P>0.05);实验组左睾丸内PCNA(669.10±205.39)mU/ml与对照组同侧(776.81±231.72)mU/ml比较下降,和实验组右睾丸(800.81±172.02)mU/ml比较也下降.差异均无统计学意义(P>0.05).结论 改进型ELV致睾丸酶活性降底和PCNA的变化,这些变化可能是影响生育能力的因素之一.
目的 觀察改進型左側精索靜脈麯張(ELV)對大鼠睪汍酶及增殖細胞覈抗原(PCNA)的影響.方法 利用改進的方法建立青春期SD大鼠左精索靜脈麯張的模型21隻;假手術組SD大鼠15隻作對照組.術後3箇月,取部分睪汍組織勻漿提取上清液,比色法定量檢測乳痠脫氫酶(LDH)、琥珀痠脫氫酶(SDH)、葡萄糖-6-燐痠脫氫酶(G6PDH)活性,利用酶聯免疫吸附試驗(ELISA)測定PCNA的濃度.結果 實驗組左睪汍內的LDH、G6PDH活性分彆為(8.17±3.47)、(34.00±16.29)U/mg,與對照組同側(11.98±2.26)、(54.88±20.87)U/mg比較下降,與實驗組右睪汍(12.69±3.97)、(78.03±25.28)U/mg比較也下降,差異均有統計學意義(P<0.05);實驗組左睪汍內的SDH活性(1.22±0.41)U/mg與對照組同側(1.74±0.43)U/mg比較下降,差異有統計學意義(P<0.05),與實驗組右睪汍(1.62±0.56)U/mg比較下降,差異無統計學意義(P>0.05);實驗組左睪汍內PCNA(669.10±205.39)mU/ml與對照組同側(776.81±231.72)mU/ml比較下降,和實驗組右睪汍(800.81±172.02)mU/ml比較也下降.差異均無統計學意義(P>0.05).結論 改進型ELV緻睪汍酶活性降底和PCNA的變化,這些變化可能是影響生育能力的因素之一.
목적 관찰개진형좌측정색정맥곡장(ELV)대대서고환매급증식세포핵항원(PCNA)적영향.방법 이용개진적방법건립청춘기SD대서좌정색정맥곡장적모형21지;가수술조SD대서15지작대조조.술후3개월,취부분고환조직균장제취상청액,비색법정량검측유산탈경매(LDH)、호박산탈경매(SDH)、포도당-6-린산탈경매(G6PDH)활성,이용매련면역흡부시험(ELISA)측정PCNA적농도.결과 실험조좌고환내적LDH、G6PDH활성분별위(8.17±3.47)、(34.00±16.29)U/mg,여대조조동측(11.98±2.26)、(54.88±20.87)U/mg비교하강,여실험조우고환(12.69±3.97)、(78.03±25.28)U/mg비교야하강,차이균유통계학의의(P<0.05);실험조좌고환내적SDH활성(1.22±0.41)U/mg여대조조동측(1.74±0.43)U/mg비교하강,차이유통계학의의(P<0.05),여실험조우고환(1.62±0.56)U/mg비교하강,차이무통계학의의(P>0.05);실험조좌고환내PCNA(669.10±205.39)mU/ml여대조조동측(776.81±231.72)mU/ml비교하강,화실험조우고환(800.81±172.02)mU/ml비교야하강.차이균무통계학의의(P>0.05).결론 개진형ELV치고환매활성강저화PCNA적변화,저사변화가능시영향생육능력적인소지일.
Objective To evaluate the effects of improved left varicocele (ELV) on testis enzymes and proliferating cell nuclear antigen (PCNA) in rats. Methods ELV models of SD rats were established by the improved method in 21 SD rats, and another 15 were included in a sham operation group as controls. At the 3rd month, the activity of LDH, SDH and G6PDH was measured by colorimetry. The concentration of the PCNA was determined by enzyme-linked immunosorbent assay ( ELISA). Results The activity of LDH and G6PDH (8. 17 ± 3. 47), (34. 00 ± 16. 29) U/mg in left testes of experimental group was reduced remarkably as compared with that in right testes (12.69 ±3.97), (78.03 ± 25. 28) U/mg and control group (11. 98 ± 2. 26) , (54. 88 ± 20. 87) U/mg (P < 0. 05). The activity of SDH was reducd remarkably in left testes of experimental group ( 1. 22 ±0. 41 ) U/mg as compared with that in control group (1.74 ± 0.43) U/mg (P < 0.05), but there was no significant difference between the left and right testes ( 1.62 ±0. 56) U/mg in experimental group. The concentration of PCNA was reduced in left testes of experimental group (669. 10 ±205. 39) mU/ml as compared with that in right testes (800. 81 ± 172. 02) mU/ml and control group (776. 81 ± 231. 72) mU/ml, but there was no significant difference (P > 0. 05). Conclusion The reduction of LDH, SDH and G6PDH and the changes of PCNA in improved ELV may play an important role in the development of subfertility.
