中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2011年
2期
101-104
,共4页
苗旺%杨期东%郭伟新%谢晓云%刘恒方%罗红波%鲍娟
苗旺%楊期東%郭偉新%謝曉雲%劉恆方%囉紅波%鮑娟
묘왕%양기동%곽위신%사효운%류항방%라홍파%포연
颅内出血,高血压性%内皮,血管%干细胞%血管内皮生长因子A
顱內齣血,高血壓性%內皮,血管%榦細胞%血管內皮生長因子A
로내출혈,고혈압성%내피,혈관%간세포%혈관내피생장인자A
Intracranial hemorrhage,hypertensive%Endothelium,vascular%Stem cells%Vascular endothelial growth factor A
目的 探讨高血压脑出血急性期患者外周血来源的内皮祖细胞(EPCs)血管内皮生长因子(VEGF)在转录、蛋白合成,以及培养上清液和血清中含量的变化.方法 分离、诱导分化高血压脑出血急性期患者和高血压患者各16例外周血EPCs,逆转录PCR方法测定EPCs VEGF mRNA表达水平,Western印迹方法检测VEGF蛋白表达水平;测定EPCs培养上清液和血清VEGF含量,并进行比较.结果 高血压脑出血组和高血压组相比,EPCs VEGF mRNA(0.186±0.035,0.090±0.031,t=8.318),蛋白表达(0.223±0.028,0.169±0.022,t=3.744),培养上清液VEGF含量[(414±37)、(316±29)pg/ml,t=8.270]和血清VEGF含量[(408±49)、(222±34)pg/ml,t=12.406]均明显增高,差异具有统计学意义(均P＜0.01).结论 脑出血急性期高血压患者EPCs VEGF的mRNA和蛋白质表达上调,EPCs和机体分泌VEGF增加.
目的 探討高血壓腦齣血急性期患者外週血來源的內皮祖細胞(EPCs)血管內皮生長因子(VEGF)在轉錄、蛋白閤成,以及培養上清液和血清中含量的變化.方法 分離、誘導分化高血壓腦齣血急性期患者和高血壓患者各16例外週血EPCs,逆轉錄PCR方法測定EPCs VEGF mRNA錶達水平,Western印跡方法檢測VEGF蛋白錶達水平;測定EPCs培養上清液和血清VEGF含量,併進行比較.結果 高血壓腦齣血組和高血壓組相比,EPCs VEGF mRNA(0.186±0.035,0.090±0.031,t=8.318),蛋白錶達(0.223±0.028,0.169±0.022,t=3.744),培養上清液VEGF含量[(414±37)、(316±29)pg/ml,t=8.270]和血清VEGF含量[(408±49)、(222±34)pg/ml,t=12.406]均明顯增高,差異具有統計學意義(均P＜0.01).結論 腦齣血急性期高血壓患者EPCs VEGF的mRNA和蛋白質錶達上調,EPCs和機體分泌VEGF增加.
목적 탐토고혈압뇌출혈급성기환자외주혈래원적내피조세포(EPCs)혈관내피생장인자(VEGF)재전록、단백합성,이급배양상청액화혈청중함량적변화.방법 분리、유도분화고혈압뇌출혈급성기환자화고혈압환자각16예외주혈EPCs,역전록PCR방법측정EPCs VEGF mRNA표체수평,Western인적방법검측VEGF단백표체수평;측정EPCs배양상청액화혈청VEGF함량,병진행비교.결과 고혈압뇌출혈조화고혈압조상비,EPCs VEGF mRNA(0.186±0.035,0.090±0.031,t=8.318),단백표체(0.223±0.028,0.169±0.022,t=3.744),배양상청액VEGF함량[(414±37)、(316±29)pg/ml,t=8.270]화혈청VEGF함량[(408±49)、(222±34)pg/ml,t=12.406]균명현증고,차이구유통계학의의(균P＜0.01).결론 뇌출혈급성기고혈압환자EPCs VEGF적mRNA화단백질표체상조,EPCs화궤체분비VEGF증가.
Objective To explore the expression of vascular endothelial growth factor (VEGF)mRNA and protein in endothelia progenitor cells (EPCs) and VEGF in the culture medium and serum of patients during acute period of cerebral hemorrhage associated with hypertension (APCHH). Methods Mononuclear cells from peripheral blood of patients with APCHH ( 16 patients) and hypertension ( 16 patients) were isolated and induced to EPCs. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed to assay VEGF mRNA. VEGF protein was assessed by Western blotting. The VEGF protein level in patient's serum and culture medium ( at day 7 ) were assayed using VEGF ELISA Kit and compared between APCHH group and hypertension group. Results Compared with hypertension group, VEGF mRNA (0. 186 ±0. 035 versus 0.090 ±0.031, t =8.318, P ＜0.0l ) and protein (0. 223 ± 0. 028 versus 0.169 ± 0. 022, t = 3. 744, P ＜ 0. 01 ) expression of EPCs, the concentration of VEGF protein in the supernatant (414 ±37 versus 316 ±29, t =8. 270, P ＜0. 01 ) and in serum (408 ±49versus 222 ±34, t = 12.406, P ＜0. 01 ) were all significantly increased in APCHH group. Conclusion The VEGF protein levels in serum of patients and in the culture medium, VEGF mRNA and protein expression in EPCs were all significantly increased during acute periods of cerebral hemorrhage.
