CAJ | 학술논문

目的：研究紫杉醇对人粘液表皮样癌细胞系MEC-1的细胞毒作用，为临床应用选择最佳给药方案提供依据。方法：应用细胞计数法研究不同药物浓度及不同作用时间细胞毒作用的特点。结果：紫杉醇对MEC-1的最大抑制率为50%～90%，当药物浓度为10 nmol/L时抑制率已接近最大，增加药物浓度10～100倍并不能增加抑制效应。但延长药物暴露时间大于12 h能明显增强抑制效应。即使作用72 h时，10～1 000 nmol/L的抑制率分别为64% 、58%、56%、44%和49%。结论：紫杉醇对人粘液表皮样癌细胞系MEC-1有明显的细胞毒作用，能明显抑制其生长，但抑制作用并不完全。此细胞毒作用的时间依赖性明显大于剂量依赖性。提示临床应用应以延长药物作用时间为目的，以期最大程度上发挥紫杉醇的抗肿瘤效应。
목적：연구자삼순대인점액표피양암세포계MEC-1적세포독작용，위림상응용선택최가급약방안제공의거。방법：응용세포계수법연구불동약물농도급불동작용시간세포독작용적특점。결과：자삼순대MEC-1적최대억제솔위50%～90%，당약물농도위10 nmol/L시억제솔이접근최대，증가약물농도10～100배병불능증가억제효응。단연장약물폭로시간대우12 h능명현증강억제효응。즉사작용72 h시，10～1 000 nmol/L적억제솔분별위64% 、58%、56%、44%화49%。결론：자삼순대인점액표피양암세포계MEC-1유명현적세포독작용，능명현억제기생장，단억제작용병불완전。차세포독작용적시간의뢰성명현대우제량의뢰성。제시림상응용응이연장약물작용시간위목적，이기최대정도상발휘자삼순적항종류효응。
Objective: To determine the sensitivity of human mucoepidermoid carcinoma MEC-1 cells to taxol.Methods:MEC-1 cells were exposed to taxol at the doses of 1,10,100 and 1 000 nmol/L;the alive cells were counted in 24,48 and 72 h respectively.In another experiment the cells were exposed to taxol at 10 and 100 nmol/L for 3,6,12,16,36,48 and 72 h respectively,then the drug was removed (except the 72 h exposure-group) and the cells were cultured with fresh medium until 72 h,and the cells were counted.Results:Immedietely after drug-exposure the inhibition effect of taxol against MEC-1 cells nearly achieved maximal(90%) at the concentration of 10 nmol/L，increasing the concentration to 10～100 fold resulted in no additional inhibition ratio in 24～72 h .However when the drug was removed after the cells had been treated at 10 and 100 nmol/L and then cultured for more than 12 hours the inhibition effect was significantly increased.Conclusion:The reaction of MEC-1 cells to Taxol depends more on exposure time than on drug concentration.