时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2001年
5期
392-393
,共2页
郑凤庚%翁连华%顾孝红%吴德康
鄭鳳庚%翁連華%顧孝紅%吳德康
정봉경%옹련화%고효홍%오덕강
RP-HPLC%明珠口服液%大黄素%含量测定
RP-HPLC%明珠口服液%大黃素%含量測定
RP-HPLC%명주구복액%대황소%함량측정
目的：建立用反相高效液相色谱(RP-HPLC)法测定明珠口服液中大黄素含量的方法。方法：采用Shim pack CLC-ODS色谱柱，流动相为甲醇-0.1%磷酸溶液(85∶15)，检测波长254 nm。结果：大黄素浓度的线性范围为0.028 32～0.141 60 μg；平均加样回收率为97.2%；RSD为1.35%(n=5)。结论：该法操作简便、快速、重现性好，可作为明珠口服液的定量分析方法。
目的：建立用反相高效液相色譜(RP-HPLC)法測定明珠口服液中大黃素含量的方法。方法：採用Shim pack CLC-ODS色譜柱，流動相為甲醇-0.1%燐痠溶液(85∶15)，檢測波長254 nm。結果：大黃素濃度的線性範圍為0.028 32～0.141 60 μg；平均加樣迴收率為97.2%；RSD為1.35%(n=5)。結論：該法操作簡便、快速、重現性好，可作為明珠口服液的定量分析方法。
목적：건립용반상고효액상색보(RP-HPLC)법측정명주구복액중대황소함량적방법。방법：채용Shim pack CLC-ODS색보주，류동상위갑순-0.1%린산용액(85∶15)，검측파장254 nm。결과：대황소농도적선성범위위0.028 32～0.141 60 μg；평균가양회수솔위97.2%；RSD위1.35%(n=5)。결론：해법조작간편、쾌속、중현성호，가작위명주구복액적정량분석방법。
Objective:To establish the method for determining the content of emodin in Mingzhu oral liquid by RP-HPLC. Methods: Using Shim pack CLC-ODS column, methanol-0.1% phosphoric acid solution (85∶15) as the mobile phase and detection wavelength at 254 nm. Results: The calibration curve was linear at a range of 0.028 32～0.141 60 μg for the emodin. The average recovery was 97.2% and the related standard deviation (RSD) was 1.35% (n=5). Conclusion:This method was proved to be simple, quick and reproducible and can be used as quantitative analysis method for this preparation.
