CAJ | 학술논문

许多研究表明,延髓头端腹外侧区(rostral ventrolateml medulla,RVLM)的NO/NOS系统参与心血管活动的中枢调节.本实验以结扎Wistar大鼠左冠状动脉前降支法建立急性心肌缺血(acute myocardial ischemia,AMI)动物模型,观察针刺"内关"穴改善AMI大鼠的心功能作用,同时检测大鼠RVLM区神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)表达的变化,进而探讨针刺治疗AMI的中枢机制.实验观察显示,AMI大鼠心功能各项指标减弱,伴随外周血去甲肾上腺素(norepinephrine,NE)和脑钠肽(brain natriuretic peptide,BNP)水平显著升高,同时RVLM区nNOS阳性神经元数和nNOS mRNA表达升高,而iNOS水平则降低.针刺"内关"穴(Pe 6)(每天30 min,连续5天)改善心功能,降低AMI大鼠血清中NE和BNP的水平,同时升高iNOS并降低nNOS在RVLM的表达.以上结果提示,针刺治疗心肌缺血的同时可以调节iNOS/NO和nNOS/NO在RVLM的变化,这可能与针刺通过调节RVLM区的NO含量进而降低交感传出,从而改善AMI大鼠的心功能有关.
허다연구표명,연수두단복외측구(rostral ventrolateml medulla,RVLM)적NO/NOS계통삼여심혈관활동적중추조절.본실험이결찰Wistar대서좌관상동맥전강지법건립급성심기결혈(acute myocardial ischemia,AMI)동물모형,관찰침자"내관"혈개선AMI대서적심공능작용,동시검측대서RVLM구신경원형일양화담합매(neuronal nitric oxide synthase,nNOS)화유도형일양화담합매(inducible nitric oxide synthase,iNOS)표체적변화,진이탐토침자치료AMI적중추궤제.실험관찰현시,AMI대서심공능각항지표감약,반수외주혈거갑신상선소(norepinephrine,NE)화뇌납태(brain natriuretic peptide,BNP)수평현저승고,동시RVLM구nNOS양성신경원수화nNOS mRNA표체승고,이iNOS수평칙강저.침자"내관"혈(Pe 6)(매천30 min,련속5천)개선심공능,강저AMI대서혈청중NE화BNP적수평,동시승고iNOS병강저nNOS재RVLM적표체.이상결과제시,침자치료심기결혈적동시가이조절iNOS/NO화nNOS/NO재RVLM적변화,저가능여침자통과조절RVLM구적NO함량진이강저교감전출,종이개선AMI대서적심공능유관.
Increasing lines of evidence has been accumulated that nitric oxide (NO) and nitric oxide synthase (NOS) distribute plentifully in the rostral ventrolateral medulla (RVLM) and contribute to cardiovascular regulation. In the present study, the expressions of neuronal and inducible isoform of NOS (nNOS and iNOS) were observed in the RVLM of acute myocardial isehemia (AMI) Wistar rats experienced electroacupuncture (EA) treatment, thereby the cardiovascular effects of NO in the RVLM were investigated and the mechanism of acupuncture effect on AMI was inferred. The results indicated that in the AMI rats, cardiac functions were markedly attenuated with high serum level of brain natriuretic peptide (BNP) and norepinephine (NE), the number of nNOS-immunoreactive cells and nNOS mRNA exprossion in the RVLM area were increased, while those of iNOS were lowered. EA at "Neiguan" aeupoints (Pe 6) 30 min daily for successive 5 d resulted in an improvement of the cardiac functions, decreases in NE and BNP levels; it also increased the expression of iNOS and decreased the expression of nNOS in the RVLM. These results suggest that the curative effect of acupuncture on AMI is possibly attributable to the differential regulation of NOS/NO in the RVLM, leading to decreased sympathetic outflow and improvement of cardiac functions.