CAJ | 학술논문

目的:探讨SHIP基因对人白血病细胞系K562细胞周期的调控作用.方法:用携带野生型SHIP基因及绿色荧光蛋白 (green fluorescent protein,GFP)基因的慢病毒及空载体慢病毒转染人白血病K562细胞.FCM检测转染效率、细胞凋亡率及细胞周期变化,实时荧光定量PCR(real-time fluorescent quantitative PCR,RFQ-PCR)检测SHIP mRNA水平的变化,Western 印迹法检测SHIP、cyclin D1、p21WAF1/CIPI、p27KIP1蛋白表达水平的变化.结果:与转染空载体组和未转染组相比,转染野生型SHIP基因的K562细胞增殖减慢,凋亡率明显上升(P＜0.05);细胞周期显示,G0/G1期延长,G1期前出现亚二倍体的凋亡峰,S期和G2/M期比例降低;cyclin D1表达降低,p21WAF1/CIPI和p27KIP1表达增加.结论:转染野生型SHIP基因可使细胞周期阻滞在G0/G1期,抑制白血病细胞增殖,促进白血病细胞凋亡.
목적:탐토SHIP기인대인백혈병세포계K562세포주기적조공작용.방법:용휴대야생형SHIP기인급록색형광단백 (green fluorescent protein,GFP)기인적만병독급공재체만병독전염인백혈병K562세포.FCM검측전염효솔、세포조망솔급세포주기변화,실시형광정량PCR(real-time fluorescent quantitative PCR,RFQ-PCR)검측SHIP mRNA수평적변화,Western 인적법검측SHIP、cyclin D1、p21WAF1/CIPI、p27KIP1단백표체수평적변화.결과:여전염공재체조화미전염조상비,전염야생형SHIP기인적K562세포증식감만,조망솔명현상승(P＜0.05);세포주기현시,G0/G1기연장,G1기전출현아이배체적조망봉,S기화G2/M기비례강저;cyclin D1표체강저,p21WAF1/CIPI화p27KIP1표체증가.결론:전염야생형SHIP기인가사세포주기조체재G0/G1기,억제백혈병세포증식,촉진백혈병세포조망.